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(γ-L-Glutamyl)putrescine and N1- and N8-(γ-L-glutamyl)spermidines, possible regioisomeric products of the polyamine glutamyl transferase of bacteria such as Escherichia coli and Pseudomonas aeruginosa, were synthesized from N-Boc-protected putrescine and 1,3-propanediamine.