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2000
Volume 21, Issue 18
  • ISSN: 1570-1808
  • E-ISSN: 1875-628X

Abstract

Background

Hepatocellular carcinoma is the cancer with the highest mortality rate worldwide. Currently, existing treatments are not very effective for this disease. Different science areas have focused on developing new therapies, including nanomedicine. studies have reported the anticancer activity of silver nanoparticles, particularly those coated with polyvinylpyrrolidone (AgNPs-PVP).

Aims

Characterize the effect of AgNPs on the HepG2 by bioinformatics analysis.

Methods

From a list of proteins, we performed analysis to predict protein-protein interaction, hub gene, gene ontology, KEGG pathways, hub gene expression, protein expression, survival, cell infiltration immune, and molecular docking of AgNPs-PVP to target proteins. Cytoscape and UCSF Chimera software, DAVID, UALCAN, TISIDB, and HDOCK databases were included in the predictive analysis.

Results

Gene ontology and KEGG pathways showed that AgNP exposure causes cellular organelles dysregulation and deregulation of protein production mechanisms.

Additionally, metabolic pathways were altered, including glycolysis, gluconeogenesis, and amino acid biosynthesis. Hub genes , , , , and showed differential expression for gene expression, protein, and survival analysis. Furthermore, and were positively correlated with CD8+ T cell infiltration, and RPLP0, EEF1B2, and were negatively correlated with NK cell infiltration. Finally, molecular docking showed that AgNPs-PVP interacts highly with the target proteins.

Conclusion

AgNPs cause alterations in cell viability. Furthermore, the deregulation of hub genes and the modulation of the immune system are associated with anticancer effects, and molecular docking demonstrated high interaction with the target proteins that should be studied experimentally.

© 2024 Bentham Science Publishers
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Bioinformatic Analysis and Molecular Docking to Elucidate the Anticancer Effect of Silver Nanoparticles in Hepatocellular Carcinoma
Letters in Drug Design & Discovery 21, 4400 (2024); https://doi.org/10.2174/0115701808352454241115065851
/content/journals/lddd/10.2174/0115701808352454241115065851
/content/journals/lddd/10.2174/0115701808352454241115065851
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  • Article Type:     Research Article
Keyword(s): Bioinformatic; cancer; HepG2; liver; molecular docking; silver nanoparticles

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