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2000
Volume 21, Issue 18
  • ISSN: 1570-1808
  • E-ISSN: 1875-628X

Abstract

Background

Mushrooms are shown to protect against the side effects of cancer. Therefore, mushrooms with proven anticancer properties and active ingredients are fascinating in the search for new cancer drugs.

Objective

In this study, the effects of extracts from (M1), (M2), (M3), (M4), and (M5) together on HCT116 were investigated. Mesenchymal stem cells (MSCs) were used to study the effect on healthy cells.

Methods

MTT was used to determine cell viability. Dose-response curves were generated, the IC values of the compounds were calculated, and the effect of the extracts was compared using it. The FTIR was used to analyze the quantitative changes of the cellular components.

Results and Discussion

The evaluation of the IC values of all fungal species showed that they reduced the cell viability of HCT116 cells. In contrast, no significant reduction in cell viability was observed in MSCs. Changes in the ratio of cell membrane lipids, proteins, and cell nucleic acids between control and fungal-treated HCT116 cells were detected by FTIR.

Conclusion

Many of the chemotherapeutic agents are of plant origin, and many resources should still be explored to inhibit the side effects of cancer therapy. The data obtained through this experiment will serve as a reference for studies to be a new source of anticancer drugs in modern pharmacology.

© 2024 Bentham Science Publishers
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Exploring the Molecular Basis of Anticancer Activity in Various Mushroom Species Against Colorectal Cancer Cells
Letters in Drug Design & Discovery 21, 4378 (2024); https://doi.org/10.2174/0115701808324049241108063414
/content/journals/lddd/10.2174/0115701808324049241108063414
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  • Article Type:     Research Article
Keyword(s): Antiproliferation; colorectal cancer; cytotoxicity; FTIR spectroscopy; mushroom; pharmaceutical research and development (R&D)

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