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2000
Volume 25, Issue 15
  • ISSN: 1568-0266
  • E-ISSN: 1873-4294

Abstract

Background

Research into oxidative stress, cancer, and natural products revealed promising avenues for therapeutic intervention. Natural products are considered potent pharmaceuticals in combating oxidative stress and its relationship with cancer.

Methods

This study was carried out to evaluate the chemical profile and antioxidant activities using DPPH, ABTS, Phenanthroline, Cupric, Phosphomolybdenum, FRAP, Hydroxyl, Iron chelation assays, and anticancer properties by MTT method of extracts. The chemical composition was determined using the LC-MS/MS technique. Therefore, methods, particularly molecular docking and dynamic simulation were applied for molecular interaction analysis.

Results

The obtained results revealed a wide variety of phenolic compounds in all studied fractions, in their qualitative and quantitative distribution. In most antioxidant assays, the butanol and ethyl acetate extracts exhibited the most effective effects, followed by the aqueous extract, while the petroleum ether and chloroform fractions exhibited much lower activity in comparison with standards. In parallel, ethyl acetate, n-butanol, and chloroform extracts exhibited potent antiproliferative activity against T47D and A549 cell lines, while the aqueous extract showed an IC in the range of mg/ml. Moreover, the analysis of interactions identified compounds against particular targets in studied cell lines using molecular docking showed a great affinity, especially for the ligands Hesperidin, Luteolin-7--glucoside and Rutin. Also, the molecular dynamic simulation of the interacting complexes Hesperidin-mTOR, Rutin-EGFR and Apigenin-HER2 revealed precise interaction, providing insights into their stability and dynamic behavior. Furthermore, the studied ligand exhibited interesting pharmacokinetic properties with no reported toxicity.

Conclusion

These findings confirmed the potential of Algerian L. leaf extracts as promising therapeutic molecules for combating oxidative stress and cancer.

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2025-11-08

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Chemical Profiling, Antioxidant, and Antiproliferative Activities of Algerian Cistus creticus L. Leaf Extracts: Evidence from In Vitro and In Silico Studies
Curr. Top. Med. Chem. 25, 1882 (2025); https://doi.org/10.2174/0115680266345222250109102407
/content/journals/ctmc/10.2174/0115680266345222250109102407
/content/journals/ctmc/10.2174/0115680266345222250109102407
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  • Article Type:     Research Article
Keyword(s): Cancer; Cistus creticus; Molecular modeling; Natural products; Oxidative stress

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