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2000
Volume 25, Issue 14
  • ISSN: 1568-0266
  • E-ISSN: 1873-4294

Abstract

Background

Zika (ZIKV) is a virus transmitted by mosquitoes that can cause Guillain-Barré syndrome and congenital malformations like microcephaly. Given its explosive resurgence and the resulting epidemics in 2016, the search for effective antiviral drugs has become absolutely necessary.

Methods

In this study, we examined the potential of naphthoquinone derivatives that have a sulfonamide or sulfonate group to inhibit ZIKV replication in primary cultured neurons and in Vero cells.

Results

In our studies, we found that PAV05 had low cytotoxicity with a CC50 of 329 µM ±3.6 for Vero cells and 290 µM ±3.5 for neurons. Additionally, we observed a strong inhibitory activity on viral replication with an EC value of EC of 0.92 µM ±0.15 in Vero cells, resulting in a Selectivity Index (SI) of 357. Even when added 16 hours post-infection, PAV05 maintained its inhibitory effect. When PAV05 was evaluated in sub-optimal concentrations together with Ribavirin, we observed a strong synergistic effect, with an inhibition greater than 90% even at doses of 0.5 µM. tests suggested that PAV05 may have effects on ZIKV NS2B-NS3.

Conclusion

The ZIKV inhibitor described in this study shows promise as a compound for the development of therapies against ZIKV. It may also be considered for inclusion in the portfolio of broad-spectrum antiflavivirus inhibitors.

© 2025 Bentham Science Publishers
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PAV-05 Naphthoquinone Potently Inhibit Zika Virus Replication in Infected Cells
Curr. Top. Med. Chem. 25, 1733 (2025); https://doi.org/10.2174/0115680266323907241212062715
/content/journals/ctmc/10.2174/0115680266323907241212062715
/content/journals/ctmc/10.2174/0115680266323907241212062715
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  • Article Type:     Research Article
Keyword(s): Antiviral; Naphthoquinone; ZIKA-Virus, Arbovirus, PAV05, NS2B-NS3

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