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2000
Volume 1, Issue 1
  • ISSN: 2772-6215
  • E-ISSN: 2772-6223

Abstract

Aim

This study aimed to evaluate the effect of Buccal Fat Pad (BFP) derived from Mesenchymal Stem Cells (MSCs) preconditioned with hypoxia on the management of diabetes mellitus in Wistar rats.

Background/Introduction

Type 1 diabetes mellitus is the most common disorder with difficult management, affecting the quality of life. Stem cell therapy has been proven to have regenerative ability. The current study has involved using the existing stem cell therapy and modifying it.

Objective

The objective of this study was to manage hyperglycemia in a diabetic rodent model by using hypoxia-preconditioned BFP-MSCs, and to study their effect on serum and pancreatic insulin and pancreatic regeneration.

Methods

In this study, the Streptozotocin (STZ)-induced diabetes rat model was used. The diabetic rats were administered the test therapy, , hypoxia-preconditioned BFP-derived MSCs in three doses by intramuscular route. Thereafter, monitoring of blood glucose levels was carried out till the end of the study. Changes in the serum insulin and pancreatic insulin were also observed. Histopathology of the pancreas was performed to assess the effect of preconditioned stem cells on pancreatic regeneration.

Results/Discussion

The effect of hypoxia-preconditioned BFP-derived MSCs on the body weight and that of food-water intake was non-significant. Their effect on blood glucose levels was found to be significant ( < 0.0001). After the administration of test therapy, the blood glucose level in the test group decreased, ultimately resulting in the management of diabetes. Histopathology of the pancreas showed regeneration of the pancreatic cells in the test group.

Conclusion

The above research findings suggest that hypoxia-preconditioned BFP-derived MSCs can be considered a promising therapy in the management of type 1 diabetes. Stem cell therapy can be the future of the management of diabetes; however, further research is needed on the current therapy.

© 2025 Bentham Science Publishers
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2024-06-12
2025-09-04

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Engineering of Mesenchymal Stem Cells in Hypoxic Condition for Pancreatic Regeneration in Diabetic Rodent Model
Curr Cell Sci 1, E230724230973 (2025); https://doi.org/10.2174/0127726215269072240524105933
/content/journals/csci/10.2174/0127726215269072240524105933
/content/journals/csci/10.2174/0127726215269072240524105933
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  • Article Type:     Research Article
Keyword(s): buccal fat pad; hypoxia; mesenchymal stem cells; streptozotocin model; Type 1 diabetes mellitus; wistar rats
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