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Chromatography is a very useful separation method that may be used for characterization, isolation/purification, and chemical and nanoparticle analysis. It has completely changed how many industries do research and development. This comprehensive study looks at its increasing importance in nanotechnology and its applications in the fields of biological sciences, pharmacology, environmental science, and industry. In the pharmaceutical business, chromatography is used to detect contaminants, guarantee drug quality, and support pharmacokinetic research. It evaluates pesticide residues, finds poisons in environmental assessments, and identifies contaminants in the air and water. Chromatography is employed in forensic science for drug testing, examination of trace evidence, and arson investigations. Chromatography is used in the food and beverage industries for quality control and taste profiling, and in the life sciences for protein separation and identification. Chromatography is used in biological research for the separation of complex mixtures and the purification of biomolecules. It is used in the industrial sector to assess petroleum products, evaluate the quality of cosmetic chemicals, and describe polymers. Because it enables the separation and analysis of nanoparticles for use in synthesis, characterization, and nanotechnology, chromatography is also becoming more and more popular in this sector. This article emphasises chromatography's enormous influence across a number of sectors as well as its developing importance in nanotechnology. Chromatographic techniques will continue to advance and change, paving the way for fresh investigations and altering the landscape of academia and business.
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