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2000
Volume 11, Issue 1
  • ISSN: 2950-4910
  • E-ISSN: 2950-4902

Abstract

Introduction

Software has been used to predict metabolic pathways for possible ‘expected’ and ‘unexpected’ metabolites based on their and fragmentation patterns from low- and high-fragmentation mass spectra of plants subjected to carbamazepine uptake has been used.

Methods

Three plants with high prevalence in the natural protected area of Doñana National Park (Spain) were irrigated with three aqueous carbamazepine solutions (10 ng‧mL-1, 700 ng‧mL,-1 and 10 μg‧mL-1). Periodically, stems, leaves, and roots were sampled and analyzed. Total carbamazepine uptake throughout the assay was evaluated and discussed, demonstrating a clear dependence on plant species/part, irrigation solution concentration, and assay duration. Additionally, chromatographic analysis was performed using a quadrupole time-of-flight (Q-TOF) mass spectrometer, recording simultaneous low and high-fragmentation mass spectra for further computational processing with MetabolynxTM software.

Results

Confirmation was achieved by analyzing the high-energy spectra obtained at the characteristic retention times and fragmentation pattern of each compound.

Discussion

Several potential carbamazepine metabolites corresponding to various metabolic pathways were identified, and, where possible, their relative abundances was discussed.

Conclusion

The use of computational tools like MetabolynxTM has proven to be useful for studies involving the identification of metabolites and/or the confirmation of metabolic pathways in the studied plants.

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Carbamazepine Uptake and Metabolization by Lavandula Dentata, Juncus Sp., and Salicornia Europaea
Curr Spectrosc Chromatogr 11, E29504910365669 (2025); https://doi.org/10.2174/0129504910365669250728013617
/content/journals/csch/10.2174/0129504910365669250728013617
/content/journals/csch/10.2174/0129504910365669250728013617
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  • Article Type:     Research Article
Keyword(s): Carbamazepine; Juncus sp.; Lavandula dentata; liquid chromatography quadrupole time-of-flight mass spectrometry; metabolites; Salicornia europaea
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