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2000
Volume 26, Issue 6
  • ISSN: 1389-2010
  • E-ISSN: 1873-4316
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Abstract

Introduction/Objective

This study aimed to examine the effect of a human umbilical cord mesenchymal stem cell-derived exosome (hUC-MSC-Exo) liquid band-aid on wound healing in mice.

Methods

hUC-MSC-Exos were prepared from the supernatant ion exchange chromatography. The composition ratio of the chitosan liquid band-aid was optimized to form a film and encapsulate hUC-MSC-Exo. The biological effects of chitosan exosome liquid band-aid on human umbilical vein endothelial cells (HUVECs) were observed, and its anti-bacterial properties were tested. BALB/c mice with back skin injury were randomly divided into chitosan exosome liquid band-aid group (CS-Exo), chitosan liquid band-aid group (CS), and normal saline control group (Con), and wound healing was evaluated post-treatment. Skin tissue samples post-treatment were collected for H&E staining.

Results

The hUC-MSC-Exo was prepared and characterized. The optimum conditions for film formation were 1% chitosan solution and 15% poloxamer 407/poloxamer 188 (pH 5.0 ~ 7.0). The chitosan exosome liquid band-aid promoted HUVEC proliferation and migration and markedly inhibited and growth . , the wound healing rate in the CS-Exo group was higher than that in the Con and CS groups. Fourteen days post-treatment, the wounds completely healed, and hair grew normally, which was consistent with H&E results. Mouse weights in each group did not change significantly after administration, indicating that the chitosan exosome liquid band-aid had no obvious toxic side effects.

Conclusion

Local chitosan exosome liquid band-aid application can promote wound healing in mice, and the mechanism could be related to hUC-MSC-Exo-induced vascular endothelial cell proliferation and migration.

© 2025 The Author(s). Published by Bentham Science Publishers. 
This is an open access article published under CC BY 4.0 https://creativecommons.org/licenses/by/4.0/legalcode
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A Liquid Band-Aid with Mesenchymal Stem Cell-derived Exosomes for Wound Healing in Mice
Current Pharmaceutical Biotechnology 26, 911 (2025); https://doi.org/10.2174/0113892010331302240913114112
/content/journals/cpb/10.2174/0113892010331302240913114112
/content/journals/cpb/10.2174/0113892010331302240913114112
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  • Article Type:     Research Article
Keyword(s): biomaterial; chitosan; exosome; Human umbilical cord; liquid band-aid; mesenchymal stem cells; wound repair

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