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2000
Volume 12, Issue 3
  • ISSN: 2213-3372
  • E-ISSN: 2213-3380

Abstract

Introduction

The study explores the biosynthesis of silver nanoparticles (AgNPs) using extracellular manganese peroxidase enzyme from Trichoderma parestonica. The synthesis was optimized at a 1:1 enzyme and silver nitrate ratio, pH 12, shaking process, and 48-hour synthesis period. The AgNPs were characterized using spectroscopic and microscopic techniques, showing absorbance in UV-spectroscopy between 410-450 nm due to Surface Plasmon Resonance (SPR).

Methods

The stabilization of extracellular manganese peroxidase with the nanoparticles through capping was observed by Fourier Transform Infrared Spectroscopy (FT-IR). The spherical shape of the AgNPs, with an average size of 69.09 nm, is confirmed by the Field Emission Scanning Electron Microscopy (FESEM) study. The size of the nanoparticles was also determined by Dynamic Light Scattering (DLS) to be 75.99 nm. When synthesized AgNPs were used to decolorize Alizarin red S (ARS), Methylene Blue (MB), and Methyl Orange (MO) in the presence of sodium borohydride reducing agent, the results showed that, within 20 minutes, 90% of 0.1 mM ARS, MB, and 75% 0.1 mM MO were degraded.

Results

This study demonstrated the potential of AgNPs synthesized from MnP enzyme in nano-remediation projects, offering a sustainable solution to the problems and issues of dye-induced wastewater pollution and fostering environmental conservation.

Conclusion

Enzymes are being studied in nanotechnology, leading to the development of enzyme nanoparticles, which can be utilized in various fields like biosensors agriculture, drug delivery, and bioremediation.

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2025-01-21
2025-09-27

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Impact of Green Synthesis of Ag-nanoparticle Using Extracellular Manganese Peroxidase on Different Dye Degradation
Curr. Organocatal. 12, 233 (2025); https://doi.org/10.2174/0122133372366582250115002303
/content/journals/cocat/10.2174/0122133372366582250115002303
/content/journals/cocat/10.2174/0122133372366582250115002303
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  • Article Type:     Research Article
Keyword(s): alizarin red S; dye decolorization; Manganese peroxidase; methyl orange; methylene blue; silver nanoparticles

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