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2000
Volume 22, Issue 3
  • ISSN: 1567-2026
  • E-ISSN: 1875-5739

Abstract

Introduction

Olfactory epithelium (OE) comprises diverse cell types, including olfactory sensory neurons (OSNs), supporting cells, and basal stem cells. While interleukin (IL)-4 is a key mediator in type 2 inflammation, its regulatory role in OE remains unclear. The current study aimed to explore the role of IL-4 in olfactory epithelial cells.

Methods

Using an olfactory epithelial organoid model, the impacts of IL-4 on different cell types were assessed by performing qPCR, immunofluorescence staining, and EdU incorporation assays. Calcium imaging was performed to assess the influence of IL-4 on OSNs, while Alcian Blue-Periodic Acid-Schiff (AB-PAS) staining was used to analyze mucin secretion in the organoids.

Results

IL-4 significantly promoted the proliferation of globular basal cells (GBCs) in basal cells, induced homeostasis of mature OSNs, and maintained the normal function of OE. However, IL-4 notably downregulated GAP43 expression in immature OSNs. Additionally, IL-4 enhanced mucin secretion in the OE.

Discussion

This study found that IL-4 promoted the differentiation of OE cells by stimulating the proliferation of GBCs and enhancing mucin secretion, while maintaining the normal function of mature olfactory neurons. Further clinical studies are needed to validate these results.

Conclusion

This study revealed the role of IL-4 in OE, providing novel insights into the mechanisms of IL-4 in inflammatory conditions.

© 2025 Bentham Science Publishers
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2025-09-25
2026-03-07

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The Role of Interleukin-4 in Olfactory Epithelial Cells: A Preliminary In Vitro Study
Curr Neurovasc Res 22, 245 (2025); https://doi.org/10.2174/0115672026420545250911091301
/content/journals/cnr/10.2174/0115672026420545250911091301
/content/journals/cnr/10.2174/0115672026420545250911091301
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  • Article Type:     Research Article
Keyword(s): basal stem cells; globular basal cells; Interleukin-4; olfactory epithelium-like organoids; olfactory function; olfactory sensory neurons

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