Optimization of Inulin Extracted with Ultrasonic-Assisted from Ornithogalum Arcuatum Liliaceae Stev. As A New Source by Response Surface Methodology

Background: Inulin is a non-digestible oligosaccharide, which can motivate the growth and activity of desired microorganisms in the colon and improve human health. Inulin serves as storage polymers and a kind of fructan in many blends containing substances. Objective: The aim of this study is optimization of conditions that affect extraction of inulin from Ornithogalum arcuatum liliaceae Stev., such as temperature, time and ratio of water to raw material. Method: In this study, RSM was used to optimize the extraction conditions of inulin from Ornithogalum arcuatum liliaceae Stev. using ultrasonic. A central composite design was used for experimental design and analysis of the results to obtain the optimal extraction conditions. Results: Three independent and main variables extraction temperature (x1: 40-100°C), extraction time (x2: 1-3 h) and ratio of water to raw material (x3: 15-25 ml g-1) were investigated to determine their importance for inulin yields. Based on the RSM analysis, optimum conditions were: temperature 70°C, time 2 h and ratio of water to raw material 20:1. Under the optimized conditions, the experimental yield was 11.895 ± 0.29%, which was in close agreement with the value of 11.838% predicted by the model. Conclusion: These results can be helpful in designing the process of optimal inulin extraction from ornithogalum arcuatum liliaceae Stev. and can be useful for the development of industrial extraction processes and reducing cost.