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2000
Volume 21, Issue 1
  • ISSN: 1573-4013
  • E-ISSN: 2212-3881

Abstract

Introduction

Fenugreek seed polysaccharides (FSP) have gained significant attention in research due to their availability in different forms, which play a tremendous role in the pharmaceutical industry as a natural excipient like matrix-former, binder, gelling, and suspending agent. Extraction of polysaccharides plays a vital role in their purity, cost, . In extraction processes, several parameters like time, temperature, pressure, solvent to be used, or the combination of all these can be modified to optimize the process.

 Aims and Objective 

The aim of this study was to optimize the extraction of the fenugreek seed polysaccharide. In other words, the objective of this study was to optimize the various parameters of the microwave-assisted extraction of polysaccharide content from the fenugreek seed () using the Box-Behnken design (BBD).

Methods

The response surface methodology (RSM) was used to optimize the extraction of the FSP. This study finds the interactive effects of the independent process variables (irradiation time, power, and solvent-to-solid ratio) on polysaccharide yield (%) by using BBD.

Results

The result showed a 40.11% yield of polysaccharides when ideal conditions were met, such as a 9.95 minute irradiation time, 51.03 solvent-to-solid ratio, and a microwave power of 180.09. The presence of functional groups in polysaccharides was confirmed by the FTIR spectrum of the isolated polysaccharide.

Conclusion

The findings demonstrate that microwave-assisted extraction is a viable method for extracting FSP.

© 2025 Bentham Science Publishers
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2025-09-21

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References

  1. ChingF.M. Chinese herbal drug research trends.Nova Publishers2007
     [Google Scholar]
  2. ZhengQ. ChenJ. YuanY. ZhangX. LiL. ZhaiY. GongX. LiB. Structural characterization, antioxidant, and anti-inflammatory activity of polysaccharides from Plumula nelumbinis.Int. J. Biol. Macromol.202221211112210.1016/j.ijbiomac.2022.05.09735594937
     [Google Scholar]
  3. KhanS. HussainA. AttarF. BloukhS.H. EdisZ. SharifiM. BalaliE. NematiF. DerakhshankhahH. ZeinabadH.A. NabiF. KhanR.H. HaoX. LinY. HuaL. ten HagenT.L.M. FalahatiM. A review of the berberine natural polysaccharide nanostructures as potential anticancer and antibacterial agents.Biomed. Pharmacother.202214611253110.1016/j.biopha.2021.11253134906771
     [Google Scholar]
  4. KalitaP. AhmedA.B. SenS. ChakrabortyR. A comprehensive review on polysaccharides with hypolipidemic activity: Occurrence, chemistry and molecular mechanism.Int. J. Biol. Macromol.202220668169810.1016/j.ijbiomac.2022.02.18935247430
     [Google Scholar]
  5. BangarB. ShindeN. DeshmukhS. KaleB. Natural polymers in drug delivery development.Res. J. Pharm. Dos. Forms Technol.201465457
     [Google Scholar]
  6. GandhiK.J. DeshmaneS.V. BiyaniK.R. Polymers in pharmaceutical drug delivery system: A review.Int. J. Pharm. Sci. Rev. Res.2012145766
     [Google Scholar]
  7. NayakA.K. PalD. SantraK. Screening of polysaccharides from tamarind, fenugreek and jackfruit seeds as pharmaceutical excipients.Int. J. Biol. Macromol.20157975676010.1016/j.ijbiomac.2015.05.01826007663
     [Google Scholar]
  8. DattaR. BandyopadhyayA. Development of a new nasal drug delivery system of diazepam with natural mucoadhesive agent from Trigonella foenum-graecum L.J. Sci. Ind. Res.20056412973977
     [Google Scholar]
  9. RashidR. AhmadH. AhmedZ. RashidF. KhalidN. Clinical investigation to modulate the effect of fenugreek polysaccharides on type-2 diabetes.Bioact Carbo Diet Fib20191910019410.1016/j.bcdf.2019.100194
     [Google Scholar]
  10. ArdilesP. Cerezal-MezquitaP. Salinas-FuentesF. ÓrdenesD. RenatoG. Ruiz-DomínguezM.C. Biochemical composition and phycoerythrin extraction from red microalgae: A comparative study using green extraction technologies.Processes2020812162810.3390/pr8121628
     [Google Scholar]
  11. ArasiM.A.S. RaoM. BagyalakshmiJ. Optimization of microwave-assisted extraction of polysaccharide from Psidium guajava L. fruits.Int. J. Biol. Macromol.20169122723210.1016/j.ijbiomac.2016.05.03927180292
     [Google Scholar]
  12. De MonteC. CarradoriS. GraneseA. Di PierroG.B. LeonardoC. De NunzioC. Modern extraction techniques and their impact on the Optimization of microwave-assisted extraction of polysaccharide from Psidium guajava L. fruits pharmacological profile of Serenoa repens extracts for the treatment of lower urinary tract symptoms.BMC Urol.20141411110.1186/1471‑2490‑14‑63
     [Google Scholar]
  13. XieJ.H. XieM.Y. ShenM.Y. NieS.P. LiC. WangY.X. Optimisation of microwave-assisted extraction of polysaccharides from Cyclocarya paliurus (Batal.) Iljinskaja using response surface methodology.J. Sci. Food Agric.20109081353136010.1002/jsfa.393520474055
     [Google Scholar]
  14. MandalV. MohanY. HemalathaS. Microwave assisted extraction—an innovative and promising extraction tool for medicinal plant research.Pharmacogn. Rev.20071718
     [Google Scholar]
  15. DahmouneF. SpignoG. MoussiK. ReminiH. CherbalA. MadaniK. Pistacia lentiscus leaves as a source of phenolic compounds: Microwave-assisted extraction optimized and compared with ultrasound-assisted and conventional solvent extraction.Ind. Crops Prod.201461314010.1016/j.indcrop.2014.06.035
     [Google Scholar]
  16. KusumaH.S. SudrajatR.G.M. SusantoD.F. GalaS. MahfudM. Response surface methodology (RSM) modeling of microwave-assisted extraction of natural dye from Swietenia mahagony: A comparation between Box-Behnken and central composite design method.AIP Conference Proceedings AIP Publishing LLC2015050009
     [Google Scholar]
  17. DeanA. VossD. Design and analysis of experiments.New YorkSpringer199910.1007/b97673
     [Google Scholar]
  18. FerreiraS.L.C. BrunsR.E. FerreiraH.S. MatosG.D. DavidJ.M. BrandãoG.C. da SilvaE.G.P. PortugalL.A. dos ReisP.S. SouzaA.S. dos SantosW.N.L. Box-Behnken design: An alternative for the optimization of analytical methods.Anal. Chim. Acta2007597217918610.1016/j.aca.2007.07.01117683728
     [Google Scholar]
  19. PavithraK. VadivukkarasiS. Evaluation of free radical scavenging activity of various extracts of leaves from Kedrostis foetidissima (Jacq.) Cogn.Food Sci. Hum. Wellness201541424610.1016/j.fshw.2015.02.001
     [Google Scholar]
  20. XieJ.H. ShenM.Y. XieM.Y. NieS.P. ChenY. LiC. HuangD.F. WangY.X. Ultrasonic-assisted extraction, antimicrobial and antioxidant activities of Cyclocarya paliurus (Batal.) Iljinskaja polysaccharides.Carbohydr. Polym.201289117718410.1016/j.carbpol.2012.02.06824750621
     [Google Scholar]
  21. SongH. ZhangQ. ZhangZ. WangJ. In vitro antioxidant activity of polysaccharides extracted from Bryopsis plumosa.Carbohydr. Polym.20108041057106110.1016/j.carbpol.2010.01.024
     [Google Scholar]
  22. DongH. ZhangQ. LiY. LiL. LanW. HeJ. LiH. XiongY. QinW. Extraction, characterization and antioxidant activities of polysaccharides of Chuanminshen violaceum.Int. J. Biol. Macromol.20168622423210.1016/j.ijbiomac.2016.01.00226806648
     [Google Scholar]
  23. LiW. CuiS. KakudaY. Extraction, fractionation, structural and physical characterization of wheat β-d-glucans.Carbohydr. Polym.200663340841610.1016/j.carbpol.2005.09.025
     [Google Scholar]
  24. Ranitha M NourA.H. ZiadA. AzhariH. Optimization of microwave assisted hydrodistillation of lemongrass (cymbopogon citratus) using response surface methodology.Int. J. Res. Eng. Technol.20143451410.15623/ijret.2014.0304002
     [Google Scholar]
  25. JingC. YuanY. TangQ. ZouP. LiY. ZhangC. Extraction optimization, preliminary characterization and antioxidant activities of polysaccharides from Glycine soja.Int. J. Biol. Macromol.20171031207121610.1016/j.ijbiomac.2017.05.18628579464
     [Google Scholar]
  26. WangW. LiX. BaoX. GaoL. TaoY. Extraction of polysaccharides from black mulberry fruit and their effect on enhancing antioxidant activity.Int. J. Biol. Macromol.2018120Pt B1420142910.1016/j.ijbiomac.2018.09.13230266643
     [Google Scholar]
  27. BourtoomT. ChinnanM.S. JantawatP. SanguandeekulR. Recovery and characterization of proteins precipitated from surimi wash-water.Lebensm. Wiss. Technol.200942259960510.1016/j.lwt.2008.09.001
     [Google Scholar]
  28. BuritiF.C.A. dos SantosK.M.O. SombraV.G. MacielJ.S. Teixeira SáD.M.A. SallesH.O. OliveiraG. de PaulaR.C.M. FeitosaJ.P.A. Monteiro MoreiraA.C.O. MoreiraR.A. EgitoA.S. Characterisation of partially hydrolysed galactomannan from Caesalpinia pulcherrima seeds as a potential dietary fibre.Food Hydrocoll.20143551252110.1016/j.foodhyd.2013.07.015
     [Google Scholar]
  29. MittalN. MattuP. KaurG. Extraction and derivatization of Leucaena leucocephala (Lam.) galactomannan: Optimization and characterization.Int. J. Biol. Macromol.20169283184110.1016/j.ijbiomac.2016.07.04627492556
     [Google Scholar]
  30. NiknamR. MousaviM. KianiH. A new source of galactomannan isolated from Gleditsia caspica (Persian honey locust) seeds: Extraction and comprehensive characterization.J. Food Process. Preserv.20214510e1577410.1111/jfpp.15774
     [Google Scholar]
  31. ChenC-W. HoC-T. Antioxidant properties of polyphenols extracted from green and black teas.J. Food Lipids199521354610.1111/j.1745‑4522.1995.tb00028.x
     [Google Scholar]
  32. MatthäusB. Antioxidant activity of extracts obtained from residues of different oilseeds.J. Agric. Food Chem.200250123444345210.1021/jf011440s12033809
     [Google Scholar]
  33. ZhaoZ.Y. ZhangQ. LiY.F. DongL.L. LiuS.L. Optimization of ultrasound extraction of Alisma orientalis polysaccharides by response surface methodology and their antioxidant activities.Carbohydr. Polym.201511910110910.1016/j.carbpol.2014.11.05225563949
     [Google Scholar]
  34. OsmanH. NasarudinR. LeeS.L. Extracts of cocoa (Theobroma cacao L.) leaves and their antioxidation potential.Food Chem.2004861414610.1016/j.foodchem.2003.08.026
     [Google Scholar]
  35. HanQ.H. LiuW. LiH.Y. HeJ.L. GuoH. LinS. ZhaoL. ChenH. LiuY.W. WuD.T. LiS.Q. QinW. Extraction optimization, physicochemical characteristics, and antioxidant activities of polysaccharides from kiwifruit (Actinidia chinensis planch.).Molecules201924346110.3390/molecules2403046130696067
     [Google Scholar]
  36. HuW. ZhaoY. YangY. ZhangH. DingC. HuC. ZhouL. ZhangZ. YuanS. ChenY. YuanM. Microwave-assisted extraction, physicochemical characterization and bioactivity of polysaccharides from Camptotheca acuminata fruits.Int. J. Biol. Macromol.201913312713610.1016/j.ijbiomac.2019.04.08630986453
     [Google Scholar]
  37. ChenY. XueY. Optimization of microwave assisted extraction, chemical characterization and antitumor activities of polysaccharides from porphyra haitanensis.Carbohydr. Polym.201920617918610.1016/j.carbpol.2018.10.09330553311
     [Google Scholar]
  38. HashemifesharakiR. XanthakisE. AltintasZ. GuoY. GharibzahediS.M.T. Microwave-assisted extraction of polysaccharides from the marshmallow roots: Optimization, purification, structure, and bioactivity.Carbohydr. Polym.202024011630110.1016/j.carbpol.2020.11630132475574
     [Google Scholar]
  39. RenB. ChenC. LiC. FuX. YouL. LiuR.H. Optimization of microwave-assisted extraction of Sargassum thunbergii polysaccharides and its antioxidant and hypoglycemic activities.Carbohydr. Polym.201717319220110.1016/j.carbpol.2017.05.09428732858
     [Google Scholar]
  40. YinC. FanX. FanZ. ShiD. GaoH. Optimization of enzymes-microwave-ultrasound assisted extraction of Lentinus edodes polysaccharides and determination of its antioxidant activity.Int. J. Biol. Macromol.201811144645410.1016/j.ijbiomac.2018.01.00729309866
     [Google Scholar]
  41. ChengZ. SongH. YangY. LiuY. LiuZ. HuH. ZhangY. Optimization of microwave-assisted enzymatic extraction of polysaccharides from the fruit of Schisandra chinensis Baill.Int. J. Biol. Macromol.20157616116810.1016/j.ijbiomac.2015.01.04825661875
     [Google Scholar]
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Optimization of Microwave-assisted Extraction of Polysaccharide from Fenugreek (Trigonella foenum-graecum) Seeds
Curr. Nutr. Food Sci. 21, 122 (2025); https://doi.org/10.2174/0115734013312926240430105914
/content/journals/cnf/10.2174/0115734013312926240430105914
/content/journals/cnf/10.2174/0115734013312926240430105914
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  • Article Type:     Research Article
Keyword(s): box-Behnken design; extraction; Fenugreek; microwave; optimization; polysaccharide

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