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2000
Volume 32, Issue 33
  • ISSN: 0929-8673
  • E-ISSN: 1875-533X

Abstract

Introduction

Elevated glucose can have a detrimental effect on the function and healing process of periodontal cells in inflammatory conditions. Hesperidin (HPN), a bioflavonoid found abundantly in citrus fruits, has numerous biological benefits, including regenerative and anti-inflammatory properties. The current study aimed to assess the impact of HPN on the proliferation, wound healing, and functionality of periodontal cells in optimal and elevated glucose conditions.

Methods

Human periodontal ligament cells (HPDLCs) were cultured in optimal glucose (1g/L) (OG) and high glucose (4.5 g/L) (HG) conditions. XTT, wound healing, ALP, and calcium release assays were conducted with or without HPN in the culture media.

Results

The statistical analysis revealed that adding different concentrations of HPN (2, 4, 10, or 100 µM) had no significant effect on the viability of HPDLCs under both OG (=0.436) and HG conditions (=0.162) compared to the control. However, in the HG condition, the addition of 100 µM HPN resulted in a statistically significant increase in wound closure (=0.003). Furthermore, in the HG condition, the addition of 100 µM HPN significantly increased ALP activity in the non-osteogenic media (=0.001) and significantly increased calcium release within the osteogenic media (=0.016).

Conclusion

The findings of this study suggest that HPN provides beneficial effects, facilitating repair and mineralization in HPDLCs under HG conditions.

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Hesperidin Improves Wound Healing and Mineralization of Periodontal Ligament Cells in Elevated Glucose Conditions
Curr. Med. Chem. 32, 7422 (2025); https://doi.org/10.2174/0109298673339671241129074005
/content/journals/cmc/10.2174/0109298673339671241129074005
/content/journals/cmc/10.2174/0109298673339671241129074005
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  • Article Type:     Research Article
Keyword(s): Diabetic culture; glucose; hesperidin; HPN; human periodontal ligament fibroblast cells; mineralization; wound healing

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