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2000
Volume 33, Issue 6
  • ISSN: 0929-8673
  • E-ISSN: 1875-533X

Abstract

Background

has traditionally been used to treat various diseases, including infections, asthma, stomach aches, and flatulence. Previous studies have highlighted its anti-inflammatory, anti-oxidative, anti-diabetic, neuroprotective, and nerve-stimulating properties.

Objective

This study aimed to evaluate the therapeutic effects and molecular mechanisms of action of the oleo-gum resin from in an animal model of diabetic neuropathy (DN).

Methods

The essential oil of oleo-gum resin from Ferula assa-foetida L. was analyzed using Gas Chromatography-Mass Spectrometric Analysis. Forty-two male Wistar rats were included in the study, with diabetes induced streptozotocin (STZ) injection. The rats were randomly assigned to seven groups (n=6 per group) and treated with different doses of extract (100, 200 mg/kg/day) or oil (10, 20 mg/kg/day), alongside appropriate control groups. After a five-week treatment period, samples of dorsal root ganglia (DRG), pancreatic tissue, and blood were collected. Key parameters assessed included blood glucose and insulin levels, motor function tests, oxidative stress protein generation, pro-inflammatory cytokine gene expression, and histopathological analyses.

Results

Treatment with various doses of extract or oil, as well as gabapentin, led to significant improvements. These included reduced blood sugar levels, increased insulin levels, and improved glycemic control. Motor function was enhanced, while the expression of pro-inflammatory cytokines and oxidative stress markers was significantly reduced.

Conclusion

These findings indicate a promising therapeutic approach for managing DN. Further studies are warranted to elucidate the underlying mechanisms of beneficial effects in DN.

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2026-02-25

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References

  1. OraiiA. ShafieeA. JalaliA. AlaeddiniF. SaadatS. MasoudkabirF. Vasheghani-FarahaniA. HeidariA. SadeghianS. BoroumandM. KarimiA. H FrancoO. Prevalence, awareness, treatment, and control of type 2 diabetes mellitus among the adult residents of tehran: Tehran cohort study.BMC Endocr. Disord.202222124810.1186/s12902‑022‑01161‑w36253738
     [Google Scholar]
  2. MaglianoD.J. BoykoE.J. IDF Diabetes Atlas.IDF Diabetes Atlas.International Diabetes FederationBrussels10th ed2021
     [Google Scholar]
  3. PapatheodorouK. BanachM. BekiariE. RizzoM. EdmondsM. Complications of diabetes 2017.J. Diabetes Res.201820181410.1155/2018/308616729713648
     [Google Scholar]
  4. FeldmanE.L. CallaghanB.C. Pop-BusuiR. ZochodneD.W. WrightD.E. BennettD.L. BrilV. RussellJ.W. ViswanathanV. Diabetic neuropathy.Nat. Rev. Dis. Primers2019514110.1038/s41572‑019‑0092‑131197183
     [Google Scholar]
  5. AhmadM.F. NaseemN. RahmanI. ImamN. YounusH. PandeyS.K. SiddiquiW.A. Naringin attenuates the diabetic neuropathy in STZ-induced type 2 diabetic wistar rats.Life20221212211110.3390/life1212211136556476
     [Google Scholar]
  6. IslamM.S. Animal models of diabetic neuropathy: Progress since 1960s.J. Diabetes Res.201320131910.1155/2013/14945223984428
     [Google Scholar]
  7. MorrowT.J. Animal models of painful diabetic neuropathy: The STZ rat modelCurr. Protoc. Neurosci.2004
     [Google Scholar]
  8. DolatabadiF. AbdolghaffariA.H. FarzaeiM.H. BaeeriM. ZiaraniF.S. EslamiM. AbdollahiM. RahimiR. The protective effect of Melissa officinalis L. in visceral hypersensitivity in rat using 2 models of acid-induced colitis and stress-induced irritable bowel syndrome: A possible role of nitric oxide pathway.J. Neurogastroenterol. Motil.201824349050110.5056/jnm1703529879761
     [Google Scholar]
  9. SodagarS. GhaneY. HeidariA. HeidariN. KhodadustE. AhmadiS.A.Y. SeirafianpourF. BaradaranH. GoodarziA. Association between metabolic syndrome and prevalent skin diseases: A systematic review and meta-analysis of case-control studies.Health Sci. Rep.202369e157610.1002/hsr2.157637752973
     [Google Scholar]
  10. DewanjeeS. DasS. DasA.K. BhattacharjeeN. DihingiaA. DuaT.K. KalitaJ. MannaP. Molecular mechanism of diabetic neuropathy and its pharmacotherapeutic targets.Eur. J. Pharmacol.201883347252310.1016/j.ejphar.2018.06.03429966615
     [Google Scholar]
  11. NasiryD. khalatbaryA.R. AhmadvandH. Talebpour AmiriF. AkbariE. Protective effects of methanolic extract of Juglans regia L. leaf on streptozotocin-induced diabetic peripheral neuropathy in rats.BMC Complement. Altern. Med.201717147610.1186/s12906‑017‑1983‑x28969623
     [Google Scholar]
  12. RoohaninasabM. MojtabaeeM. LivaniF. HeidariN. AlizadehN. KhosraviS. GoodarziA. Beneficial esthetic lightening effects of Cuscuta extract on skin darkness in healthy individuals: A clinical trial study.J. Family Med. Prim. Care202211116890689510.4103/jfmpc.jfmpc_783_2236993036
     [Google Scholar]
  13. WHO traditional medicine strategy: 2014-2023.World Health Organization2013
     [Google Scholar]
  14. SchippmannU. LeamanD. CunninghamA. A comparison of cultivation and wild collection of medicinal and aromatic plants under sustainability aspects.Frontis2006759510.1007/1‑4020‑5449‑1_6
     [Google Scholar]
  15. ChenW. LuoY.F. LiuJ.P. Topical herbal medicine for treatment of diabetic peripheral neuropathy: A systematic review of randomized controlled trials.Forsch. Komplement. Med.201118313414510.1159/00032845721701182
     [Google Scholar]
  16. ChenX.M. LinG.X. WangX. MaH.Y. WangR.S. WangS.M. TangD. Beneficial effects of ginsenosides on diabetic nephropathy: A systematical review and meta- analysis of preclinical evidence.J. Ethnopharmacol.2023302Pt A11586010.1016/j.jep.2022.11586036341813
     [Google Scholar]
  17. Homayouni MoghadamF. DehghanM. ZarepurE. DehlaviR. GhaseminiaF. EhsaniS. MohammadzadehG. BarzegarK. Oleo gum resin of Ferula assa-foetida L. ameliorates peripheral neuropathy in mice.J. Ethnopharmacol.2014154118318910.1016/j.jep.2014.03.06924709312
     [Google Scholar]
  18. RaafatK. El-LakanyA. Acute and subchronic in-vivo effects of Ferula hermonis L. and Sambucus nigra L. and their potential active isolates in a diabetic mouse model of neuropathic pain.BMC Complement. Altern. Med.201515125710.1186/s12906‑015‑0780‑726220172
     [Google Scholar]
  19. XuH.B. JiangR.H. ChenX.Z. LiL. Chinese herbal medicine in treatment of diabetic peripheral neuropathy: A systematic review and meta-analysis.J. Ethnopharmacol.2012143270170810.1016/j.jep.2012.07.03422885132
     [Google Scholar]
  20. IranshahyM. IranshahiM. Traditional uses, phytochemistry and pharmacology of asafoetida (Ferula assa-foetida oleo-gum-resin)—A review.J. Ethnopharmacol.2011134111010.1016/j.jep.2010.11.06721130854
     [Google Scholar]
  21. Al-AwadiF.M. KhattarM.A. GumaaK.A. On the mechanism of the hypoglycaemic effect of a plant extract.Diabetologia198528743243410.1007/BF002808863899826
     [Google Scholar]
  22. LatifiE. MohammadpourA.A. HB.F. NouraniH. Antidiabetic and antihyperlipidemic effects of ethanolic Ferula assa-foetida oleo-gum-resin extract in streptozotocin-induced diabetic wistar rats.Biomed. Pharmacother.201911019720210.1016/j.biopha.2018.10.15230471513
     [Google Scholar]
  23. MahendraP. BishtS. Ferula asafoetida : Traditional uses and pharmacological activity.Pharmacogn. Rev.201261214114610.4103/0973‑7847.9994823055640
     [Google Scholar]
  24. FarzaeiM.H. RahimiR. AttarF. SiavoshiF. SanieeP. HajimahmoodiM. MirnezamiT. KhanaviM. Chemical composition, antioxidant and antimicrobial activity of essential oil and extracts of Tragopogon graminifolius, a medicinal herb from Iran.Nat. Prod. Commun.2014911934578X140090013410.1177/1934578X140090013424660479
     [Google Scholar]
  25. BasuS. DasM. SenA. ChoudhuryU.R. DattaG. Analysis of complete nutritional profile and identification of bioactive components present in Alocasia indica tuber cultivated in Howrah District of West Bengal, India.Asian Pac. J. Trop. Med.201471S527S533
     [Google Scholar]
  26. NiazK. HassanF.I. MabqoolF. KhanF. MomtazS. BaeeriM. Navaei-NigjehM. RahimifardM. AbdollahiM. Effect of styrene exposure on plasma parameters, molecular mechanisms and gene expression in rat model islet cells.Environ. Toxicol. Pharmacol.201754627310.1016/j.etap.2017.06.02028688303
     [Google Scholar]
  27. SchmittgenT.D. LivakK.J. Analyzing real-time PCR data by the comparative CT method.Nat. Protoc.2008361101110810.1038/nprot.2008.7318546601
     [Google Scholar]
  28. RichnerM. JagerS.B. SiupkaP. VaegterC.B. Hydraulic extrusion of the spinal cord and isolation of dorsal root ganglia in rodents.J. Vis. Exp.2017119e5522610.3791/55226‑v28190031
     [Google Scholar]
  29. WangH. JosephJ.A. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader11Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee by the United States Department of Agriculture and does not imply its approval to the exclusion of other products that may be suitable.Free Radic. Biol. Med.1999275-661261610.1016/S0891‑5849(99)00107‑010490282
     [Google Scholar]
  30. BenzieI.F.F. StrainJ.J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay.Anal. Biochem.19962391707610.1006/abio.1996.02928660627
     [Google Scholar]
  31. BădescuS.V. TătaruC.P. KobylinskaL. GeorgescuE.L. ZahiuD.M. ZăgreanA.M. ZăgreanL. Effects of caffeine on locomotor activity in streptozotocin-induced diabetic rats.J. Med. Life20169327527927974933
     [Google Scholar]
  32. ForoumadiR. BaeeriM. AsgarianS. EmamgholipourZ. GoliF. FiroozpourL. KeykhaeiM. GholamiM. DehpourA.R. AbdollahiM. ForoumadiA. Antidiabetic and neuroprotective effects of a novel repaglinide analog.J. Biochem. Mol. Toxicol.2022369e2312510.1002/jbt.2312535702883
     [Google Scholar]
  33. SchmeichelA.M. SchmelzerJ.D. LowP.A. Oxidative injury and apoptosis of dorsal root ganglion neurons in chronic experimental diabetic neuropathy.Diabetes200352116517110.2337/diabetes.52.1.16512502508
     [Google Scholar]
  34. Abu-ZaitonA.S. Anti-diabetic activity of Ferula assafoetida extract in normal and alloxan-induced diabetic rats.Pak. J. Biol. Sci.20101329710010.3923/pjbs.2010.97.10020415145
     [Google Scholar]
  35. TakeokaG. Volatile constituents of Asafoetida.ACS Publications200110.1021/bk‑2001‑0794.ch004
     [Google Scholar]
  36. HoferO. WidhalmM. GregerH. Circular dichroism of sesquiterpene-umbelliferone ethers and structure elucidation of a new derivative isolated from the gum resin “Asa Foetida”.Monatsh. Chem.1984115101207121810.1007/BF00809352
     [Google Scholar]
  37. Seyed MohammadN. DehpourA.A. EbrahimzadehM.A. Seyed FazelN. Antioxidant activity of the methanol extract of Ferula assafoetida and its essential oil composition.Grasas Aceites200960440541210.3989/gya.010109
     [Google Scholar]
  38. KhajehM. YaminiY. BahramifarN. SefidkonF. Reza PirmoradeiM. Comparison of essential oils compositions of Ferula assa-foetida obtained by supercritical carbon dioxide extraction and hydrodistillation methods.Food Chem.200591463964410.1016/j.foodchem.2004.06.033
     [Google Scholar]
  39. NabaviS.M. EbrahimzadehM.A. NabaviS.F. EslamiB. DehpourA.A. Antioxidant and antihaemolytic activities of Ferula foetida regel (Umbelliferae).Eur. Rev. Med. Pharmacol. Sci.201115215716421434482
     [Google Scholar]
  40. ElsnerM. GuldbakkeB. TiedgeM. MundayR. LenzenS. Relative importance of transport and alkylation for pancreatic beta-cell toxicity of streptozotocin.Diabetologia200043121528153310.1007/s00125005156411151762
     [Google Scholar]
  41. RafieeS. MojadadiM.S. MolaviM. NazemiS. Effect of ethyl acetate extract of ferula asafoetida oleo-gum resin on the glucose level and lipid profile in streptozotocin-induced diabetic rats.Jundishapur J. Nat. Pharm. Prod.2019152e6704210.5812/jjnpp.67042
     [Google Scholar]
  42. DongJ. ZhangX. ZhangL. BianH.X. XuN. BaoB. LiuJ. Quercetin reduces obesity-associated ATM infiltration and inflammation in mice: A mechanism including AMPKα1/SIRT1.J. Lipid Res.201455336337410.1194/jlr.M03878624465016
     [Google Scholar]
  43. SrinivasanM. SudheerA.R. MenonV.P. Ferulic acid: Therapeutic potential through its antioxidant property.J. Clin. Biochem. Nutr.20074029210010.3164/jcbn.40.9218188410
     [Google Scholar]
  44. IranshahiM. AlizadehM. Antihyperglycemic effect of asafoetida (Ferula assafoetida Oleo-Gum-Resin)in streptozotocin-induced diabetic rats.World Appl. Sci. J.201217157162
     [Google Scholar]
  45. JainS. PandhiP. SinghA.P. MalhotraS. Efficacy of standardized herbal extracts in type 1 diabetes - An experimental study.Afr. J. Tradit. Complement. Altern. Med.2006342333
     [Google Scholar]
  46. YusufogluH.S. SolimanG.A. RahmanR.F.A. Abdel-KadeM.S. GanaieM.A. BedirE. ErelS.B. OzturkB. Anti-hyperglycemic and antihyperlipidemic effects of Ferula assa-foetida and Ferula tenuissima extracts in diabetic rats.Pak. J. Biol. Sci.201518731432310.3923/pjbs.2015.314.323
     [Google Scholar]
  47. GhorbaniA. Jalili-NikM. SoukhtanlooM. JavanshirS. Jahani YazdiA. EsmaeilizadehM. JafarianA.H. Effects of ethanolic extract of Ferula gummosa oleo-resin in a rat model of streptozotocin-induced diabetes.Res. Pharm. Sci.201914213814510.4103/1735‑5362.25336131620190
     [Google Scholar]
  48. KamiyaH. ZhangmW. SimaA.A.F. Apoptotic stress is counterbalanced by survival elements preventing programmed cell death of dorsal root ganglions in subacute type 1 diabetic BB/Wor rats.Diabetes200554113288329510.2337/diabetes.54.11.328816249457
     [Google Scholar]
  49. KamiyaH. ZhangW. SimaA.A.F. Degeneration of the Golgi and neuronal loss in dorsal root ganglia in diabetic BioBreeding/Worcester rats.Diabetologia200649112763277410.1007/s00125‑006‑0379‑017047923
     [Google Scholar]
  50. YeB. WangS. ZhangL. Studies on the detoxification effects and acute toxicity of a mixture of cis -sec-butyl-1-propoenyl disulphide and trans -sec-butyl-1-propoenyl disulphide isolated from crude essential oil of Ferula sinkiangensis K.M. Shen, a Chinese traditional herbal medicine.Nat. Prod. Res.201125121161117010.1080/14786419.2010.55002721740281
     [Google Scholar]
  51. LorenzettiB.B. SouzaG.E.P. SartiS.J. Santos FilhoD. FerreiraS.H. Myrcene mimics the peripheral analgesic activity of lemongrass tea.J. Ethnopharmacol.1991341434810.1016/0378‑8741(91)90187‑I1753786
     [Google Scholar]
  52. LeeS.C. TsaiC.C. YaoC.H. ChenY.S. WuM.C. Ferulic acid enhances peripheral nerve regeneration across long gaps.Evid. Based Complement. Alternat. Med.201320131810.1155/2013/87632723690861
     [Google Scholar]
  53. ChoH.I. KimK.M. KwakJ.H. LeeS.K. LeeS.M. Protective mechanism of anethole on hepatic ischemia/reperfusion injury in mice.J. Nat. Prod.20137691717172310.1021/np400432323962021
     [Google Scholar]
  54. SilvaA.C.R. LopesP.M. AzevedoM.M.B. CostaD.C.M. AlvianoC.S. AlvianoD.S. Biological activities of a-Pinene and β-Pinene enantiomers.Molecules20121766305631610.3390/molecules1706630522634841
     [Google Scholar]
  55. VincentA.M. RussellJ.W. LowP. FeldmanE.L. Oxidative stress in the pathogenesis of diabetic neuropathy.Endocr. Rev.200425461262810.1210/er.2003‑001915294884
     [Google Scholar]
  56. DaneshkazemiA. ZandiH. DavariA. VakiliM. EmtiaziM. LotfiR. MasoumiS.M.R. Antimicrobial activity of the essential oil obtained from the seed and Oleo-Gum-Resin of Ferula Assa-Foetida against oral pathogens.Front. Dent.201916211312010.18502/fid.v16i2.136231777852
     [Google Scholar]
  57. KavoosiG. RowshanV. Chemical composition, antioxidant and antimicrobial activities of essential oil obtained from Ferula assa-foetida oleo-gum-resin: Effect of collection time.Food Chem.201313842180218710.1016/j.foodchem.2012.11.13123497874
     [Google Scholar]
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The Protective Effects of Ferula assa-foetida L. oleo-gum Resin on Diabetic Neuropathy in Animal Models
Curr. Med. Chem. 33, 1233 (2026); https://doi.org/10.2174/0109298673327506250305043440
/content/journals/cmc/10.2174/0109298673327506250305043440
/content/journals/cmc/10.2174/0109298673327506250305043440
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  • Article Type:     Research Article
Keyword(s): anti-oxidative; Diabetes mellitus; diabetic neuropathy; Ferula assa-foetida L.; oleo-gum resin; streptozotocin

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