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2000
Volume 22, Issue 8
  • ISSN: 1567-2018
  • E-ISSN: 1875-5704

Abstract

Background

Eczema, an inflammatory skin disease causing intense itching, is a function of a range of internal and external factors, impacting individuals of all ages and leading to economic loss. Inflammation is the most important manifestation of eczema, and Matricaria recutita essential oil (MREO) extracted from Matricaria recutita possesses excellent antibacterial and anti-inflammatory properties.

Methods

In this study, Matricaria recutita microemulsions were prepared by the trans-phase emulsification method and their stability was determined by evaluating the relevant indexes. Establishment of 2,4-dinitro-chlorobenzene-induced AD model in mice. Detection of serum indexes of IL-6, IL-17, and TNF-α, and on pathological tissue sections, the HE staining, toluidine blue staining, immunohistochemistry, and observation were performed.

Results

The study obtained optimal conditions for the preparation of microemulsion formulations of Matricaria recutita. Through quality evaluation, it was found that the microemulsion increased stability, reduced irritation, and retained anti-inflammatory activity and therapeutic effects on eczema compared to Matricaria recutita essential oil (MREO). Studies have demonstrated that microemulsion formulations of Matricaria recutita and Matricaria recutita significantly down regulate the pro-inflammatory factors TNF-α, IL-17, and IL-6. It was shown by hematoxylin-eosin (HE) staining that both Matricaria recutita essential oil (MREO) and Matricaria recutita microemulsion (MRME) improved the inflammatory status of eczematous skin tissues in mice. The number of mast cells expressed in the tissues was decreased in the surface-treated group, as shown by toluidine blue staining. Additionally, the number of mast cells expressed in the tissues in the surface-treated group was reduced, as demonstrated by immunohistochemistry. Furthermore, immunohistochemistry revealed that MREO and MRME have immunomodulatory effects on the tissues.

Conclusion

The study showed that microemulsion formulations of Matricaria recutita may serve as a novel remedy for eczema.

© 2025 Bentham Science Publishers
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2025-09-03
2026-02-19

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References

  1. SohnA. FrankelA. PatelR.V. GoldenbergG. Eczema.Mt. Sinai J. Med.201178573073910.1002/msj.2028921913202
     [Google Scholar]
  2. LeungA.K.C. LamJ.M. LeongK.F. LeungA.A.M. WongA.H.C. HonK.L. Nummular Eczema: An Updated Review.Recent Pat. Inflamm. Allergy Drug Discov.202014214615510.2174/22122710MTA59MDEa032778043
     [Google Scholar]
  3. TianS BaiM MiaoMS KangL Analysis of eczema animal model based on clinical characteristics.Zhongguo Zhong Yao Za Zhi.201843485385610.19540/j.cnki.cjcmm.20171027.024
     [Google Scholar]
  4. MorenoA. Renert-YuvalY. Guttman-YasskyE. Shedding light on key pharmacological knowledge and strategies for pediatric atopic dermatitis.Expert Rev. Clin. Pharmacol.202316211913110.1080/17512433.2023.217317236705936
     [Google Scholar]
  5. AkdisC.A. AkdisM. BoydS.D. SampathV. GalliS.J. NadeauK.C. Allergy: Mechanistic insights into new methods of prevention and therapy.Sci. Transl. Med.202315679eadd256310.1126/scitranslmed.add256336652536
     [Google Scholar]
  6. AdcockI.M. MumbyS. Glucocorticoids.Handb. Exp. Pharmacol.201623717119610.1007/164_2016_9827864677
     [Google Scholar]
  7. El MihyaouiA. Esteves da SilvaJ.C.G. CharfiS. Candela CastilloM.E. LamartiA. ArnaoM.B. Chamomile (Matricaria chamomilla L.): A Review of Ethnomedicinal Use, Phytochemistry and Pharmacological Uses.Life (Basel)202212447910.3390/life1204047935454969
     [Google Scholar]
  8. EgbutaM.A. McIntoshS. WatersD.L.E. VancovT. LiuL. In vitro anti-inflammatory activity of essential oil and β-bisabolol derived from cotton gin trash.Molecules202227252610.3390/molecules2702052635056836
     [Google Scholar]
  9. LiG XuX ZuoYY Langmuir-Blodgett transfer from the oil-water interface.J Colloid Interface Sci.2023630Pt B212710.1016/j.jcis.2022.10.063
     [Google Scholar]
  10. ChenX. LiX. LuH. XuY. WeiY. CaoK. ZhuZ. ChenM. YuW. Mouse Model of Critical Persistent Inflammation, Immunosuppression, and Catabolism Syndrome.Shock202257223824510.1097/SHK.000000000000187834678913
     [Google Scholar]
  11. TartaroG. MateosH. SchironeD. AngelicoR. PalazzoG. Microemulsion Microstructure(s): A Tutorial Review.Nanomaterials (Basel)2020109165710.3390/nano1009165732846957
     [Google Scholar]
  12. ChevalierR.C. GomesA. CunhaR.L. Role of aqueous phase composition and hydrophilic emulsifier type on the stability of W/O/W emulsions.Food Res. Int.202215611112310.1016/j.foodres.2022.11112335651003
     [Google Scholar]
  13. SinghO. KhanamZ. MisraN. SrivastavaM. Chamomile (Matricaria chamomilla L.): An overview.Pharmacogn. Rev.201159829510.4103/0973‑7847.7910322096322
     [Google Scholar]
  14. PerazzoA. PreziosiV. GuidoS. Phase inversion emulsification: Current understanding and applications.Adv. Colloid Interface Sci.201522258159910.1016/j.cis.2015.01.00125632889
     [Google Scholar]
  15. GomesA. FurtadoG.F. CunhaR.L. Bioaccessibility of Lipophilic Compounds Vehiculated in Emulsions: Choice of Lipids and Emulsifiers.J. Agric. Food Chem.2019671131810.1021/acs.jafc.8b0546030556391
     [Google Scholar]
  16. ChouhanS. ChauhanL.S. Effect of Surfactant Chain Length on Emulsification Dynamics of Self Emulsifying Formulation of Poorly Soluble Drug.Curr. Drug Deliv.202219887488810.2174/156720181866621072709263934315366
     [Google Scholar]
  17. HungC.F. HwangT.L. ChangC.C. FangJ.Y. Physicochemical characterization and gene transfection efficiency of lipid emulsions with various co-emulsifiers.Int. J. Pharm.20052891-219720810.1016/j.ijpharm.2004.11.00815652212
     [Google Scholar]
  18. YangN. MaoP. LvR. ZhangK. FangY. NishinariK. PhillipsG.O. Stability and Oil Migration of Oil‐in‐Water Emulsions Emulsified by Phase‐Separating Biopolymer Mixtures.J. Food Sci.2016818E1971E198010.1111/1750‑3841.1338427384744
     [Google Scholar]
  19. SubiratsX. YuanH.P. ChavesV. MarzalN. RosésM. Microemulsion electrokinetic chromatography as a suitable tool for lipophilicity determination of acidic, neutral, and basic compounds.Electrophoresis201637142010201610.1002/elps.20160008027126602
     [Google Scholar]
  20. BachuRD StepanskiM AlzhraniRM JungR BodduSHS Development and evaluation of a novel microemulsion of dexamethasone and tobramycin for topical ocular administration.J Ocul Pharmacol Ther.201834431232410.1089/jop.2017.0082
     [Google Scholar]
  21. LiM. ZhangH. WuZ. ZhuZ. JiaX. DPD Simulation on the Transformation and Stability of O/W and W/O Microemulsions.Molecules2022274136110.3390/molecules2704136135209153
     [Google Scholar]
  22. PengL. Study on improving the stability of Antelope Pearl San based on the concept of "medicine and auxiliary in one" by introducing Pickering milk technology.Shaanxi University of Traditional Chinese Medicine202310.27294/d.cnki.gsxzc.2023.000040
     [Google Scholar]
  23. RiedlR. KühnA. RietzD. HebeckerB. GlowallaK.G. PeltnerL.K. JordanP.M. WerzO. LorkowskiS. WiegandC. WallertM. Establishment and Characterization of Mild Atopic Dermatitis in the DNCB-Induced Mouse Model.Int. J. Mol. Sci.202324151232510.3390/ijms24151232537569701
     [Google Scholar]
  24. HanifinJM BaghoomianW GrinichE LeshemYA JacobsonM SimpsonEL The Eczema Area and Severity Index-A Practical Guide.Dermatitis202233318719210.1097/DER.0000000000000895
     [Google Scholar]
  25. DuanJ.W. LiJ.K. WangY.J. ZhouP.J. WangX. XiaN. WangJ. LiJ. WangW.F. WangX. SunJ. GuoD.Y. ZouJ.B. ZhangX.F. WangC.L. Therapeutic effects and mechanism of action of lavender essential oil on atopic dermatitis by modulating the STAT3/RORγt pathway.Arab. J. Chem.202417210552510.1016/j.arabjc.2023.105525
     [Google Scholar]
  26. GanS.D. PatelK.R. Enzyme immunoassay and enzyme-linked immunosorbent assay.J. Invest. Dermatol.201313391310.1038/jid.2013.28723949770
     [Google Scholar]
  27. ZhangW. TangR. BaG. LiM. LinH. Anti-allergic and anti-inflammatory effects of resveratrol via inhibiting TXNIP-oxidative stress pathway in a mouse model of allergic rhinitis.World Allergy Organ. J.2020131010047310.1016/j.waojou.2020.10047333133334
     [Google Scholar]
  28. AkajiS. SagawaT. HondaA. MiyasakaN. SadakaneK. IchinoseT. TakanoH. Post-staining Raman analysis of histological sections following decolorization.Analyst (Lond.)2022147204473447910.1039/D2AN01138G36062919
     [Google Scholar]
  29. VidalB.C. MelloM.L.S. Toluidine blue staining for cell and tissue biology applications.Acta Histochem.2019121210111210.1016/j.acthis.2018.11.00530463688
     [Google Scholar]
  30. Ramos-VaraJ.A. Technical aspects of immunohistochemistry.Vet. Pathol.200542440542610.1354/vp.42‑4‑40516006601
     [Google Scholar]
  31. LairikyengbamD. WetterauerB. SchmiechM. JahrausB. KirchgessnerH. WetterauerP. BerschneiderK. BeierV. NieslerB. BaltaE. SamstagY. Comparative analysis of whole plant, flower and root extracts of Chamomilla recutita L. and characteristic pure compounds reveals differential anti-inflammatory effects on human T cells.Front. Immunol.202415138896210.3389/fimmu.2024.138896238720895
     [Google Scholar]
  32. RaveendranR. Tips and Tricks for Controlling Eczema.Immunol. Allergy Clin. North Am.201939452153310.1016/j.iac.2019.07.00631563186
     [Google Scholar]
  33. LeeY. ChoiH.K. N’dehK.P.U. ChoiY.J. FanM. KimE. ChungK.H. AnJ.H. Inhibitory Effect of Centella asiatica Extract on DNCB-Induced Atopic Dermatitis in HaCaT Cells and BALB/c Mice.Nutrients202012241110.3390/nu1202041132033291
     [Google Scholar]
  34. HuY. WangJ. ZhangH. XieH. SongW. JiangQ. ZhaoN. HeS. Enhanced Expression of IL-18 and IL-18BP in Plasma of Patients with Eczema: Altered Expression of IL-18BP and IL-18 Receptor on Mast Cells.Mediators Inflamm.2017201711210.1155/2017/309078228839348
     [Google Scholar]
  35. BaiX.Y. LiuP. ChaiY.W. WangY. RenS.H. LiY.Y. ZhouH. Artesunate attenuates 2, 4-dinitrochlorobenzene-induced atopic dermatitis by down-regulating Th17 cell responses in BALB/c mice.Eur. J. Pharmacol.202087417302010.1016/j.ejphar.2020.17302032087254
     [Google Scholar]
/content/journals/cdd/10.2174/0115672018315617240826133041
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Studies on the Preparation of a Microemulsion Formulation of Matricaria Recutita Essential Oil and the Treatment of 2,4-Dinitro-Chlorobenzene-Induced Eczema in Mice by Inhibiting Inflammation
Curr. Drug Deliv. 22, 1180 (2025); https://doi.org/10.2174/0115672018315617240826133041
/content/journals/cdd/10.2174/0115672018315617240826133041
/content/journals/cdd/10.2174/0115672018315617240826133041
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  • Article Type:     Research Article
Keyword(s): eczema; immunohistochemistry; inflammatory factor; Matricaria recutita; O/W microemulsion; pseudo-ternary phase diagram

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