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image of The Regulatory Effects of Electroacupuncture on the Intestinal Flora of Mice with Ulcerative Colitis

Abstract

Introduction

This study aimed to investigate the modulation of intestinal flora by electroacupuncture in a murine ulcerative colitis (UC) model, with a focus on analyzing microbial taxa and identifying key regulatory targets and pathways.

Methods

A UC model was established in mice using 5% dextran sodium sulfate (DSS). Electroacupuncture was applied at bilateral “Shangjuxu” (electrostimulation) and “Tianshu” (manual acupuncture) points from days 5–9, while the mesalazine group received 0.5 g/kg/day via gavage. Disease activity index (DAI), colon length, and histopathology (hematoxylin-eosin staining) were evaluated. Intestinal flora composition was analyzed 16S rDNA sequencing.

Results

Electroacupuncture significantly reduced DAI scores on days 7 and 9 ( < 0.05; < 0.01) compared to the model group, improved colon morphology, and reduced inflammation. Linear discriminant analysis and Wilcoxon tests revealed an increased abundance of and elevated alpha diversity in the electroacupuncture group. Functional prediction demonstrated suppressed RNA transport and glycerophospholipid metabolism in the model group ( < 0.05), which were significantly enhanced post-electroacupuncture ( < 0.01).

Discussion

Electroacupuncture restored beneficial taxa (., ) and microbial diversity, suggesting gut homeostasis modulation. Enhanced lipid metabolism and RNA transport pathways may underlie its anti-inflammatory and mucosal repair effects.

Conclusion

Electroacupuncture alleviates UC by modulating the structure and function of intestinal flora, with and associated metabolic pathways identified as key targets. These findings highlight the potential of electroacupuncture as a UC therapy.

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2025-04-07
2025-09-14

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The Regulatory Effects of Electroacupuncture on the Intestinal Flora of Mice with Ulcerative Colitis
Bentham Science Publishers ; https://doi.org/10.2174/0113862073357858250219050156
/content/journals/cchts/10.2174/0113862073357858250219050156
/content/journals/cchts/10.2174/0113862073357858250219050156
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Supplements

Supplementary material is available on the publisher’s website along with the published article. Supplemental Table 1: DAI scoring criteria. Supplemental Table 2: Analysis of community composition. Supplemental Table 3: Venn diagram showing C, EA, M, and mesalazine common community. Supplemental Table 4: Statistical table of difference test for the C group . the M group and the M group . the EA group.

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  • Article Type:     Research Article
Keywords: Electroacupuncture ; RNA transport ; ulcerative colitis ; lipid metabolism ; intestinal flora ; Roseburia

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