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2000
Volume 28, Issue 17
  • ISSN: 1386-2073
  • E-ISSN: 1875-5402

Abstract

Background

Diabetes mellitus (DM) is a chronic metabolic disease. The leaves of Lour. (LYY), a well-known traditional Chinese medicine (TCM) with Guangxi national characteristics often used in simple recipes to treat DM has attracted increasing attention. In this study, we investigated the therapeutic effects of LYY in diabetic rats from a metabolomic perspective.

Methods

The type 2 diabetes (T2DM) rat model was induced by a high-sugar and high-fat diet (HSFD) combined with 40 mg/kg streptozotocin (STZ). After oral administration of LYY (10.7 g/kg) for 28 d, their weight, fasted blood glucose (FBG), blood lipid levels, and inflammatory factors were assessed. The feces, urine, and serum samples of the rats were collected, and proton nuclear magnetic resonance (1H-NMR) technology was used to explore the changes in the sample's metabolism spectrum and analyze the relevant targeted metabolic pathways.

Results

Compared with the diabetes group, LYY rats significantly delayed the reduction of body weight and decreased the FBG level ( <0.01); the levels of total cholesterol (TC), triglycerides (TG), and low-density lipoprotein-cholesterol (LDL-C), IL-6, and TNF-α in serum significantly reduced ( < 0.05, 0.01), and the level of high-density lipoprotein-cholesterol (HDL-C) significantly increased ( < 0.01). 2 candidate biomarkers were identified from feces samples, and 4 associated metabolic pathways were discovered. 13 potential biomarkers were screened from urine samples, leading to the identification of 16 related metabolic pathways. Similarly, 5 potential biomarkers were screened from serum samples, and 11 related metabolic pathways were found.

Conclusion

LYY can regulate the metabolic disorder caused by T2DM by regulating amino acid metabolism, amino acid synthesis, and tricarboxylic acid cycle, which provides a specific reference for the clinical treatment of T2DM.

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2025-02-03
2025-12-24

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Study on the Mechanism of the Leaves of Dimocarpus longan Lour. in the Management of Type 2 Diabetes based on Metabolomics
Comb Chem High Throughput Screen 28, 3105 (2025); https://doi.org/10.2174/0113862073335304241023153906
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Attribution was made according to Chenomx NMR suite 8.6 software, using the BMRB website and compound structure knowledge. The two-dimensional 1H-1H COSY identification results of different metabolites in feces, urine, and serum of different groups are shown in Fig. S1-S3.

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  • Article Type:     Research Article
Keyword(s): hyperglycemia; metabonomic; proton nuclear magnetic resonance; streptozotocin; The leaves of Dimocarpus longan Lour.; type 2 diabetes

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