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image of Mechanism of Qilong Capsule against Myocardial Ischemia-Reperfusion Injury Based on Network Pharmacology and Experimental Validation

Abstract

Introduction

Qilong capsule (QC) has been used clinically to treat ischemic stroke in China. This study evaluated the therapeutic effects of QC on myocardial ischemia-reperfusion injury (MIRI) and its potential mechanisms.

Method

The components and candidate targets of QC against MIRI were predicted by network pharmacology relevant databases such as TCMSP, BATMAN-TCM, GeneCards. The potential mechanisms were predicted by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses and verified by enzyme-linked immunosorbent assay (ELISA) and Western blot.

Results

Network pharmacology analysis indicated that the cardioprotective effect of QC against MIRI was associated with inflammatory pathways. We further confirmed that QC effectively decreased the levels of inflammatory factors, including hs-CRP and MCP-1, and suppressed the expression of TNF-α and the phosphorylation of STAT3.

Conclusion

This study provides evidence for further clinical applications of QC for MIRI therapy.

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2025-01-10
2025-08-19

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Mechanism of Qilong Capsule against Myocardial Ischemia-Reperfusion Injury Based on Network Pharmacology and Experimental Validation
Bentham Science Publishers ; https://doi.org/10.2174/0113862073332431241120044411
/content/journals/cchts/10.2174/0113862073332431241120044411
/content/journals/cchts/10.2174/0113862073332431241120044411
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  • Article Type:     Research Article
Keywords: STAT3 ; qilong capsule ; heart ; Chinese medicine ; myocardial ischemia-reperfusion injury ; inflammation

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