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2000
Volume 28, Issue 14
  • ISSN: 1386-2073
  • E-ISSN: 1875-5402

Abstract

Background

Glioblastoma (GBM) severely disrupts the quality of life of patients. Anoikis represents a significant mechanism in cancer invasion and metastasis. Our study focused on the prognostic relationship between the anoikis-associated gene and GBM and its effect on GBM cell progression.

Methods

We downloaded 656 and 979 GBM sample data from TCGA and CGGA cohort datasets, respectively. Fifteen anoikis-associated genes were obtained from the GeneCards database and were subsequently clustered to identify differential genes associated with them. After univariate analysis and survival random forest dimensionality reduction, the anoikis score was the sum of the coefficients * gene expression values of the five prognostic genes. Survival analysis and ROC curve analysis of anoikis scores were performed using the TCGA training and CGGA validation sets. The prognostic factors were analyzed using Cox regression analysis in GBM. Moreover, CCK-8, colony formation, wound healing, and transwell assay were used to evaluate GBM cell proliferation and migration.

Results

Significant differences were observed in the 5-year survival of GBM patients between the two subgroups. Then, our analysis demonstrated that high OCIAD2, FTLP3, IGFBP2, and H19 levels were associated with lower 5-year GBM survival rates, whereas high SFRP2 levels were associated with higher survival rates. Univariate Cox analysis indicated that GBM risk was linked to both anoikis score and grade, while multivariate Cox analysis indicated that GBM risk was associated with both anoikis score and age. Additionally, OCIAD2 was highly expressed in U251MG and T98G cells. Moreover, OCIAD2 silencing inhibited GBM cell proliferation and migration.

Conclusion

This study demonstrated the potential of the anoikis-associated gene OCIAD2 as a prognostic biomarker for GBM. Furthermore, we validated that OCIAD2 promoted GBM cell progression. OCIAD2 silencing inhibited GBM cell proliferation and migration.

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Knockdown of Anoikis-Associated Gene OCIAD2 Reduces Proliferation and Migration of Glioblastoma Cell Lines
Comb Chem High Throughput Screen 28, 2477 (2025); https://doi.org/10.2174/0113862073305357240719070044
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Table . Raw data of clinical characteristics. The table contained information on data, such as grade, gender, and age. It was used to analyze the correlation between anoikis-associated genes and clinical characteristics. Annex . STR analysis of LN229, T98G, U87MG, and U251MG cells.

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  • Article Type:     Research Article
Keyword(s): anoikis score; GBM; migration; OCIAD2; proliferation; TCGA

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