Optimization and Characterization of Biogenic Silver Nanoparticles Synthesized by Leaves Extract of Alphonsea madraspatana

Background: Literature evidence as well as traditional uses of genus Alphonsea reveal significant antimicrobial and anti-oxidant activitiesencouraging to consider A. madraspatana to have potent antimicrobials, there by offering potential adjuncts to synthesize improved antimicrobial Silver nanoparticles (AgNPs). The objective of the present exposition is to optimize reaction parameters to synthesize antimicrobial Biogenic Silver nanoparticles (BAgNPs) from the extract of A. madraspatana leaves (AML) and to evaluate the effect against bacteria. Methods: BAgNPs were synthesized by the optimized reaction. The Synthesized nanoparticles were characterized by UV, IR, ICP-MS and XRD analysis. The antibacterial potency of optimized BAgNPs was evaluated against E. coli by comparing with positive controls. Results: Results of the optimization process indicate nanoscale BAgNPs were produced at 45°C for 120 min at pH 8 with 1:5 volume ratio of AgNO3 and extract. Optimized BAgNPs exhibits relatively higher antimicrobial activity (31±1mm) compared to Ciprofloxacin (27±1mm) and marketed nanosilver (28± 2 mm). The developed BAgNPs show comparable biofilm inhibition (86.50%) as compared to marketed nanosilver (88.10%) and Ciprofloxacin (83.10%). Conclusion: Experimental evidence suggests methanolic extract of AML under predefined conditions, which successfully generate nano-template of silver with better antibacterial response against E. coli.