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2000
Volume 22, Issue 1
  • ISSN: 1573-4072
  • E-ISSN: 1875-6646

Abstract

Background

In recent years, chemical and biological studies have explored medicinal plants to develop new treatments for oxidative stress, inflammation, and diabetes.

Objective

This study aims to characterize for the first time the chemical composition of the hexane extract from the roots of using GC and GC/MS techniques. We also isolated its major compound and evaluated its biological activities, including its antioxidant, anti-inflammatory, antidiabetic, and hemolytic effects, as well as the combination of this major compound with reference drugs.

Methods

Antioxidant activity was assessed using DPPH, -carotene, and phosphomolybdenic tests. The anti-inflammatory effect was measured by the egg albumin denaturation method and protein denaturation inhibition. The antidiabetic activity was determined by evaluating the inhibition of α-amylase, with acarbose as a positive reference. The combined effect of aethiopinone with standards was also studied to reduce the minimum effective dose and minimize side effects.

Results

The hexane extract of is mainly composed of aethiopinone (53.3%) and ferruginol (20.5%). aethiopinone was isolated and identified using spectroscopic methods, including 1H NMR, 13C NMR, and IR. The biological activity assessment showed that hexane extract and aethiopinone had promising antioxidant, anti-inflammatory, and antidiabetic properties. In addition, synergistic effects were observed when aethiopinone was combined with positive references, resulting in a significant reduction of the required minimum inhibitory concentrations. The human erythrocyte toxicity assessment showed that hexane extract and aethiopinone induced very low hemolysis levels, reaching 24.18% and 31.13%, respectively, at high concentrations of 2000 μg/mL.

Conclusion

Further research is needed to confirm their therapeutic effects and assess their potential for use in the pharmaceutical industry.

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Study of the Biological Activities of Aethiopinone Isolated from the Hexane Extract of Salvia argentea L. Roots and its Combinations with Reference Products
Curr. Bioact. Compd. 22, E15734072356174 (2026); https://doi.org/10.2174/0115734072356174241228013353
/content/journals/cbc/10.2174/0115734072356174241228013353
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  • Article Type:     Research Article
Keyword(s): aethiopinone; biological activities; GC/MS techniques; hemolytic activity; Salvia argentea; α-amylase

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