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2000
Volume 21, Issue 4
  • ISSN: 1573-4072
  • E-ISSN: 1875-6646

Abstract

Background

To understand the effectiveness of identified bioactive compounds derived from as antibacterial agents against infections was studied. Antimicrobial resistance (AMR) has put many modern treatments aside and proved ineffective over many human infections. The misuse and inappropriate overuse of antimicrobials in humans have developed drug-resistant pathogens called “superbugs.” Currently, the World is facing a rise in the crisis regarding strategic information and innovation with a novel breakthrough on demand with an efficient, safe, and alternative drug to check severe attacks of AMR. Herbal medicines are now being pipelined with substantial understanding and trials in complete treatment for many AMR infections. This study is an innovative approach that shows constant stability by directly inhibiting the protein involved in cell wall synthesis of the pathogen with the ligand selected on interaction.

Objectives

i) Elucidating the bidirectional approach of bioactive compounds identified from as an alternative drug to control infection caused by AMR . ii) Molecular dynamics simulation analysis to prove its significance in understanding the mechanism of penicillin-binding protein 2a responsible for cell wall synthesis in AMR being inhibited by the bioactive compounds more effectively than FDA-approved drugs. iii) The bioactive compounds selected were studied for antibacterial and anti-inflammatory activities.

Methods

A molecular docking study was performed using AMdock v1.5.2 with the PBP2a from S. SwissADME v2023 and pass online v2.0 were used to understand the drug-likeness prophecies for these compounds. MD simulations identified Beta-Glucogallin (BEG) and Dihydro Dehydro Coniferyl alcohol (DIH) as hit compounds. Using GROMACS v2020.6, BEG and DIH, identified for molecular dynamics simulation studies, indicated maximum hydrogen bonds. All the results of RMSD, RMSF, SASA, RG, H bonding, and MMPBSA were compared and analyzed with FDA-approved drugs. The phytocompounds present in were retrieved from the PubMed database.

Results

Binding affinities for DIH and BEG; -55.202 +/-20.494, and 27.972+/-16.329 with 1MWU respectively. The RMSD (1MWU-APO, 1MWU-MET, 1MWU-BEG and 1MWU-DIH complex proteins were 0.860 +/- 0.04 nm, 0.80 +/- 0.08 nm, 0.56 +/- 0.07 nm, 0.51 +/- 0.10 nm respectively). RMSF (1MWU-APO, 1MWU-MET, 1MWU-BEG and 1MWU-DIH complex proteins were 0.27 +/- 0.14 nm, 0.20 +/- 0.09 nm, 0.29 +/- 0.16 nm, 0.29 +/- 0.14 nm respectively). RG (1MWU-APO, 1MWU-MET, 1MWU-BEG and 1MWU-DIH complex proteins were 3.47 +/- 0.09 nm, 3.45 +/- 0.05, 3.66 +/- 0.04 nm, 3.63 +/- 0.06 nm respectively). SASA (1MWU-APO, 1MWU-MET, 1MWU-BEG and 1MWU-DIH) complex proteins were 312.26 +/- 7.43 nm, 319.68 +/- 4.46, 321.16 +/- 4.85 nm, 338.4 +/- 4.05 nm respectively.

Conclusion

The computational analysis utilized molecular docking and molecular dynamics simulations to evaluate interactions between , Penicillin-binding protein 2a (PBP2a), and phytochemical compounds from . Among the compounds analyzed, Beta-Glucogallin (BEG) and Dihydro Dehydro Coniferyl alcohol (DIH) exhibited stable binding and interactions, demonstrating potential as antimicrobial agents. PASS software analysis further supported their antibacterial and anti-inflammatory activities, indicating the need for subsequent and validation studies. These compounds show promise as leads for drug development efforts to address antimicrobial resistance.

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Molecular Dynamics Simulation Studies for the Antibacterial Activity of Bioactive Compound from Syzygium cumini against AMR Staphylococcus aureus
Curr. Bioact. Compd. 21, e270624231370 (2025); https://doi.org/10.2174/0115734072309616240613071448
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  • Article Type:     Research Article
Keyword(s): beta-glucogallin; dihydro dehydro coniferyl alcohol; PBP2a; Staphylococcus aureus; superbugs; Syzygium cumini

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