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2000
Volume 21, Issue 4
  • ISSN: 1573-4072
  • E-ISSN: 1875-6646

Abstract

Objectives

The purpose of this research was to determine whether or not Chaturbeeja Churnam (CC) had anti-arthritic properties and the effect it has on Wistar rats that have been induced with arthritis using Complete Freund's Adjuvant (CFA).

Methods

Injection of 0.2 milliliters of CFA into the sub-plantar surface of the left hind paw resulted in the development of arthritis. The test samples CC-1 and CC-2 were administered to the animals for a period of 21 days in a row. After the arthritis was induced, the rise in swelling that was noticed was observed. The blood samples that were taken were then used in additional testing with a hematology analyzer to determine the number of red blood cells (RBC), white blood cells (WBC), erythrocytes sedimentation rate (ESR), and hemoglobin (Hb). Using rat ELISA kits, the levels of IL-6 and TNF- that were found in the serum were also analyzed.

Results

CC showed a significant inhibitory effect in the protein denaturation assay. Results showed that a significant reduction in paw edema was observed in CC-2 treated rats. The maximum anti-inflammatory activity (59.12%) of the CC was noticed at a dose level of 400 mg/kg. The paw edema was restored on day 21 was 4.52 mm for CC-2, which is near to the control group. The arthritis score in treated rats was found to be considerably lower than in the control group 0.92 for CC-2 and 1.61 for CC-1. A decrease in levels of RBC and hemoglobin were observed in arthritic rats. Inflammation was significantly reduced and serum levels of IL-6 and TNF-α were lowered after treatment with the test drug.

Conclusion

It can be concluded from the study that CC possess significant anti-arthritic activity. Furthermore, this condition was linked to a reduction in abnormal humoral immune responses.

© 2025 Bentham Science Publishers
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2024-06-05
2025-10-12

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Anti-arthritic Activity and Anti-inflammatory Mediators Modulation Effect of Bioactive Traditional Chaturbeeja Churna on Arthritis Experimental Model
Curr. Bioact. Compd. 21, e050624230692 (2025); https://doi.org/10.2174/0115734072301905240523102653
/content/journals/cbc/10.2174/0115734072301905240523102653
/content/journals/cbc/10.2174/0115734072301905240523102653
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  • Article Type:     Research Article
Keyword(s): arthritis; Ayurvedic; collagen; ELISA; ethnic medicine; interleukin; TNF-α

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