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2000
Volume 22, Issue 1
  • ISSN: 1573-4110
  • E-ISSN: 1875-6727

Abstract

Objectives

This is the first report on the development and validation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC)-densitometric methods for the identification of linoleic acid (LA) in petroleum ether extract (PEE) of (EN) stem (ST), latex (LX), and bark (BA).

Methodology

Chromatographic analyses were performed on silica gel-G and silica gel 60 F plates.

Results

The chromatographic analyses revealed better spots and well-separated peaks of LA with retention factor (R) values at 0.54 (ST), 0.40 (LX), and 0.64 (BA), respectively. The linearity of the calibration curve ranges from 10-50 ng/spot (ST), 10-100 ng/spot (LX), and 50-200 ng/spot (BA). The proposed method was characterized by better accuracy, better robustness, and good precision, ranging from 0.173 to 0.372% (intra-day) and 0.185 to 0.205% (inter-day). The value of the limit of detection and quantification equal to 1.04 and 3.16 ng/spot in ST, 0.87 and 2.64 ng/spot in LX, and 0.177 and 0.53 ng/spot in BA determined the sensitivity of the method. In the obtained chromatogram, no peak was observed other than the LA which determined the specificity of the method. The % RSD of < 2% after periods of 12, 24, 36, 48, and 72 h determined the stability of standard LA.

Conclusion

Thus, the fingerprinting method is valuable in determining the adulterants and in routine quality control of formulations and herbal drugs.

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Isolation, Development and Validation of Chromatographic Methods for the Estimation of Linoleic Acid from Different Parts of Euphorbia neriifolia Linn.
Current Analytical Chemistry 22, 63 (2026); https://doi.org/10.2174/0115734110337548241003080952
/content/journals/cac/10.2174/0115734110337548241003080952
/content/journals/cac/10.2174/0115734110337548241003080952
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  • Article Type:     Research Article
Keyword(s): densitometry; Derivization; Euphorbia neriifolia; HPTLC; linoleic acid; TLC

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