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2000
Volume 21, Issue 4
  • ISSN: 1573-4110
  • E-ISSN: 1875-6727

Abstract

Background

, a traditional Chinese herbal medicine, contains abundant volatile components and effective ingredients such as flavonoids, exerting anti-inflammatory, antioxidant and antibacterial effects. The pharmacologically active components are differentially distributed in different parts of . Leaf loss commonly occurs during the processing of medicinal and edible products.

Methods

In the present study, the total flavonoid content and DPPH antioxidant activity of the stems and leaves of were compared and analyzed. A method for determining the content of six antioxidant-active components in stems and leaves was established based on high-performance liquid chromatography. Simultaneously, the contents of volatile compounds in stems and leaves were determined using gas chromatography–mass spectrometry, and the compositional differences between stem and leaf extracts of were analyzed.

This study aims to develop and validate an analytical method based on combined HPLC and GC-MS analysis of the constituents of , and to compare the differences in constituents in different tissues of using the method.

Results

The average total flavonoid content in the leaves of the eight batches of was 0.4 mg/mL, while the average total flavonoid content in the stems was 0.3 mg/mL. The average DPPH scavenging rate of the leaves in the eight batches of was 99.35%, while the average DPPH scavenging rate of the stems was 98.88%. According to the results of the HPLC assay, the content of flavonoids is significantly higher in leaves than in stems. Kaempferol was not detected in stem samples from various batches. The highest percentage of terpenoids was found in the leaves by GC-MS assay.

Conclusion

The medicinal components were concentrated in the leaves of , which could contribute to cost savings, increase production efficiency, and promote the development of the industry.

© 2025 Bentham Science Publishers
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2024-09-04
2025-09-06

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Polygonum hydropiper Leaves have More Medicinal Value than Stems: Based on Chemical Composition and Antioxidant Activity
Current Analytical Chemistry 21, 407 (2025); https://doi.org/10.2174/0115734110326836240820113411
/content/journals/cac/10.2174/0115734110326836240820113411
/content/journals/cac/10.2174/0115734110326836240820113411
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  • Article Type:     Research Article
Keyword(s): antioxidant; compositional differences; content determination; GC-MS; HPLC; Polygonum hydropiper; volatile components

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