Skip to content
2000
Volume 21, Issue 8
  • ISSN: 1573-4110
  • E-ISSN: 1875-6727

Abstract

Objective

The objective of this study is to develop a novel fluorometric aptasensor employing fluorescence resonance energy transfer (FRET) for the detection of Cadmium (II) (Cd2+) in water and food samples. The constructed aptasensor employed a fluorophore-quencher labeled aptamer combination not previously reported for Cd2+ detection. Additionally, its simple mix-and-detect pattern without immobilization or material-assisted steps represented an innovative design.

Methods

Utilizing 6-carboxyfluorescein (FAM)-modified aptamers and maleimide (BHQ-1)-modified aptamer complementary chain to construct a fluorescent detection probe, this aptasensor achieved a rapid, sensitive, and selective detection of Cd2+. Without Cd2+, the aptamer and its complementary strand undergo base pairing, bringing the FAM closer to the BHQ-1, and leading to FRET and a subsequent decrease in fluorescence intensity. The introduction of Cd2+ preferentially brought to the aptamer, changing its conformation and preventing the quenching of FAM by BHQ-1, thereby restoring the fluorescence intensity of the aptasensor.

Results

Following optimization of experimental parameters, the aptasensor exhibited a linear response to Cd2+ concentrations ranging from 5 to 1200 nM, with a detection limit (LOD) of 0.43 nM. The aptasensor’s performance was unaffected by the presence of various ions, indicating its high specificity. Moreover, it could rapidly and accurately detect Cd2+ in water and food samples, including tap water, lake water, grapes, cabbage, and broccoli, demonstrating its substantial potential for practical application.

Conclusion

Therefore, the developed aptasensor represents an important tool for effective Cd2+ detection in water and food matrices, highlighting its potential as a critical tool for environmental monitoring and food safety.

© 2025 Bentham Science Publishers
Loading

Article metrics loading...

/content/journals/cac/10.2174/0115734110319398240715050604
2024-07-25
2025-12-24

  • From This Site

    /content/journals/cac/10.2174/0115734110319398240715050604
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

References

  1. ZhuJ. WangD. YuH. YinH. WangL. ShenG. GengX. YangL. FeiY. DengY. Advances in colorimetric aptasensors for heavy metal ion detection utilizing nanomaterials: A comprehensive review.Anal. Methods202315466320634310.1039/D3AY01815F 37965993
     [Google Scholar]
  2. NirmalaN. ShrinitiV. AasreshaK. ArunJ. GopinathK.P. DawnS.S. SheeladeviA. PriyadharsiniP. BirindhadeviK. ChiN.T.L. PugazhendhiA. Removal of toxic metals from wastewater environment by graphene-based composites: A review on isotherm and kinetic models, recent trends, challenges and future directions.Sci. Total Environ.202284015656410.1016/j.scitotenv.2022.156564 35690214
     [Google Scholar]
  3. HaripriyanU. ArunJ. GopinathK.P. MythiliR. KimW. GovarthananM. A mini-review on innovative strategies for simultaneous microbial bioremediation of toxic heavy metals and dyes from wastewater.Arch. Microbiol.202320512910.1007/s00203‑022‑03367‑x 36522563
     [Google Scholar]
  4. ArunJ. SushmaR. DarshanB.S. PandimadeviM. Chemically enhanced coffee husks as bio-sorbents for the removal of copper and nickel ions from aqueous solutions: Study on kinetic parameters.Desalination Water Treat.201812129130410.5004/dwt.2018.22510
     [Google Scholar]
  5. MaY. WangL. CaoY. LiangT. WangP. LuoH. YuJ. ZhangD. XingB. YangB. Stabilization and remediation of heavy metal-contaminated soils in China: Insights from a decade-long national survey.Environ. Sci. Pollut. Res. Int.20222926390773908710.1007/s11356‑021‑18346‑w 35098461
     [Google Scholar]
  6. ZhangY. LiangR. ChenY. WangY. LiX. WangS. JinH. LiuL. TangZ. HSF1 protects cells from cadmium toxicity by governing proteome integrity.Ecotoxicol. Environ. Saf.202326611557110.1016/j.ecoenv.2023.115571 37837696
     [Google Scholar]
  7. HussainT. GondalM.A. Detection of toxic metals in waste water from dairy products plant using laser induced breakdown spectroscopy.Bull. Environ. Contam. Toxicol.200880656156510.1007/s00128‑008‑9418‑5 18414762
     [Google Scholar]
  8. HouY. JiaB. ShengP. LiaoX. ShiL. FangL. ZhouL. KongW. Aptasensors for mycotoxins in foods: Recent advances and future trends.Compr. Rev. Food Sci. Food Saf.20222122032207310.1111/1541‑4337.12858 34729895
     [Google Scholar]
  9. AfrasiabiS. PourhajibagherM. RaoofianR. TabarzadM. BahadorA. Therapeutic applications of nucleic acid aptamers in microbial infections.J. Biomed. Sci.2020271610.1186/s12929‑019‑0611‑0 31900238
     [Google Scholar]
  10. GaoZ. WangY. WangH. LiX. XuY. QiuJ. Recent aptamer-based biosensors for Cd2+ detection.Biosensors (Basel)202313661210.3390/bios13060612 37366977
     [Google Scholar]
  11. GuoY. ZhangY. ShaoH. WangZ. WangX. JiangX. Label-free colorimetric detection of cadmium ions in rice samples using gold nanoparticles.Anal. Chem.201486178530853410.1021/ac502461r 25117533
     [Google Scholar]
  12. LiY. RanG. LuG. NiX. LiuD. SunJ. XieC. YaoD. BaiW. Highly sensitive label-free electrochemical aptasensor based on screen-printed electrode for detection of Cadmium (II) ions.J. Electrochem. Soc.20191666B449B45510.1149/2.0991906jes
     [Google Scholar]
  13. ChenS. ZhuS. HeY. LuD. Speciation of chromium and its distribution in tea leaves and tea infusion using titanium dioxide nanotubes packed microcolumn coupled with inductively coupled plasma mass spectrometry.Food Chem.201415025425910.1016/j.foodchem.2013.10.150 24360447
     [Google Scholar]
  14. GuptaR. KaulS. SinghV. KumarS. SinghalN.K. Graphene oxide and fluorescent aptamer based novel biosensor for detection of 25-hydroxyvitamin D3.Sci. Rep.20211112345610.1038/s41598‑021‑02837‑4 34873222
     [Google Scholar]
  15. LiuH. GaoY. MathivananJ. Armour-GarbZ. ShaoZ. ZhangY. ZhaoX. ShaoQ. ZhangW. YangJ. CaoC. LiH. ShengJ. GanJ. Crystal structures and identification of novel Cd2+-specific DNA aptamer.Nucleic Acids Res.20235194625463610.1093/nar/gkad239 37013991
     [Google Scholar]
  16. GuanH. YangS. ZhengC. ZhuL. SunS. GuoM. HuX. HuangX. WangL. ShenZ. DNAzyme-based sensing probe protected by DNA tetrahedron from nuclease degradation for the detection of lead ions.Talanta202123312254310.1016/j.talanta.2021.122543 34215046
     [Google Scholar]
  17. LiuY. ZhangD. DingJ. HayatK. YangX. ZhanX. ZhangD. LuY. ZhouP. A facile aptasensor for instantaneous determination of Cadmium ions based on fluorescence amplification effect of MOPS on FAM-labeled aptamer.Biosensors (Basel)202111513310.3390/bios11050133 33922514
     [Google Scholar]
  18. HassanS.A.E.M. AhmedS.A.E.F. HelmyA.H. YoussefN.F. Spectrofluorimetric study on fluorescence quenching of tyrosine and L ‐tryptophan by the aniracetam cognition enhancer drug: Qquenching mechanism using Stern–Volmer and double‐log plots.Luminescence202035572873710.1002/bio.3778 31994341
     [Google Scholar]
  19. HanK. ChenL. ZhangW. TongY. ShiJ. SuX. ZouX. A ratiometric electrochemical sensor for detecting lead in fish based on the synergy of semi-complementary aptamer pairs and Ag nanowires@zeolitic imidazolate framework-8.Anal. Methods202315182199220910.1039/D3AY00196B 37114376
     [Google Scholar]
  20. ImranM. AnwarK. AkramM. ShahG.M. AhmadI. Samad ShahN. KhanZ.U.H. RashidM.I. AkhtarM.N. AhmadS. NawazM. SchottingR.J. Biosorption of Pb(II) from contaminated water onto Moringa oleifera biomass: Kinetics and equilibrium studies.Int. J. Phytoremediation201921877778910.1080/15226514.2019.1566880 31081349
     [Google Scholar]
  21. QiuZ. ShuJ. TangD. Bioresponsive release system for visual fluorescence detection of carcinoembryonic antigen from mesoporous silica nanocontainers mediated optical color on quantum dot-enzyme-impregnated paper.Anal. Chem.20178995152516010.1021/acs.analchem.7b00989 28376620
     [Google Scholar]
  22. DaiQ. LiuW. ZhuangX. WuJ. ZhangH. WangP. Ratiometric fluorescence sensor based on a pyrene derivative and quantification detection of heparin in aqueous solution and serum.Anal. Chem.201183176559656410.1021/ac2008724 21800849
     [Google Scholar]
  23. ZhenJ. LiangG. ChenR. JiaW. Label-free hairpin-like aptamer and EIS-based practical, biostable sensor for acetamiprid detection.PLoS One20201512e024429710.1371/journal.pone.0244297 33362222
     [Google Scholar]
  24. WHO. Guidelines for drinking-water quality, 4th edition, incorporating the 1st addendum.2017Available From: https://www.who.int/publications/i/item/9789241549950
    [Google Scholar]
  25. NehzatiS. SummersA.O. DolgovaN.V. ZhuJ. SokarasD. KrollT. PickeringI.J. GeorgeG.N. Hg(II) binding to thymine bases in DNA.Inorg. Chem.202160107442745210.1021/acs.inorgchem.1c00735 33938732
     [Google Scholar]
  26. YuH. PanC. ZhuJ. ShenG. DengY. XieX. GengX. WangL. Selection and identification of a DNA aptamer for fluorescent detection of netilmicin.Talanta202225012370810.1016/j.talanta.2022.123708 35752088
     [Google Scholar]
/content/journals/cac/10.2174/0115734110319398240715050604
Loading
A Simple Aptasensor Based on Fluorescence Resonance Energy Transfer for the Rapid Detection of Cadmium (II) In Water and Food
Current Analytical Chemistry 21, 1005 (2025); https://doi.org/10.2174/0115734110319398240715050604
/content/journals/cac/10.2174/0115734110319398240715050604
/content/journals/cac/10.2174/0115734110319398240715050604
Loading

Data & Media loading...

Supplements

Supplementary material is available on the publisher's website along with the published article.

file format download Download

  • Article Type:     Research Article
Keyword(s): Cadmium (II); detection limit; detection range; fluorescence resonance energy transfer; fluorometric aptasensor; safety analysis

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test