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image of Extraction and Characterization of Iturin A as a Key Factor in the Antagonism of Bacillus amyloliquefaciens M13RW01 Toward Pathogenic Fungi

Abstract

Background

contains several fungal inhibitory compounds, such as peptides and lipopeptides, representing the remarkable potential for biotechnological, agricultural, and biopharmaceutical applications.

Objectives

This research aimed to extract and characterize iturin A as the key antagonism factor of M13RW01 toward pathogenic fungi, using HPLC and mass spectrometry (MS) analysis.

Methods

For this study, strain M13-RW01 isolated from Isfahan soil was used. The lipopeptide compounds of were examined for antagonistic performance against PTCC 5010, PTCC 5292, CBS 62087, and PTCC 5037 by well diffusion and percentage of growth inhibition. The crude extract was run on Waters μBondpak C18 column in the HPLC system to separate the antibiotics. Major antibiotics were analyzed based on MS.

Results

HPLC analysis demonstrated that the lipopeptide compound is similar to iturin A. Moreover, MS analysis of these compounds and purified iturin A revealed a high similarity between them, with the same molecular ion peaks identified. Results showed that the produced lipopeptides by were of iturin A genum. The molecular ion peaks of the M13RW01 methanolic fraction were at 1027.10, 1043.05, 1058, 1066, 1072, 1088.95. These compounds restrained fungal germination and growth. Inhibition growth percentages were 79.28, 76.13, 84.47and 59.15% for , s, and , respectively.

Conclusion

According to the present study, M13RW01 lipopeptides are able to inhibit the growth of some fungi. M13-RW01 isolated from Isfahan soil plays an essential part in antagonizing pathogenic fungi. Thus, this antifungal lipopeptide is supposed to be a biological protection agent for agricultural products and patents.

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2025-04-16
2025-09-27

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/content/journals/biot/10.2174/0118722083352297250326041149
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Extraction and Characterization of Iturin A as a Key Factor in the Antagonism of Bacillus amyloliquefaciens M13RW01 Toward Pathogenic Fungi
Bentham Science Publishers ; https://doi.org/10.2174/0118722083352297250326041149
/content/journals/biot/10.2174/0118722083352297250326041149
/content/journals/biot/10.2174/0118722083352297250326041149
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  • Article Type:     Research Article
Keywords: fungal germination ; lipopeptide ; iturin ; mass spectrometry ; Bacillus amyloliquefaciens ; methanolic fraction ; farm crops ; pathogenic fungi

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