Recent Advances in CRISPR-Cas Genome Engineering: An Overview
- Authors: Angelina Job Kolady1, Aritra Mukherjee2, Ranjith Kumavath3, Sarvepalli Vijay Kumar4, Pasupuleti Sreenivasa Rao5
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View Affiliations Hide Affiliations1 Department of Genomic Science, School of Biological Sciences, Central University of Kerala, Tejaswini Hills, Periya (PO) Kasaragod, Kerala 671320, India 2 Department of Genomic Science, School of Biological Sciences, Central University of Kerala, Tejaswini Hills, Periya (PO) Kasaragod, Kerala 671320, India 3 Department of Genomic Science, School of Biological Sciences, Central University of Kerala, Tejaswini Hills, Periya (PO) Kasaragod, Kerala 671320, India 4 Department of Paediatrics, Lincoln University College, Kuala Lumpur, Malaysia 5 Central Research Laboratory (ARC), Narayana Medical College and Hospital, Nellore 524003, India
- Source: Genome Editing in Bacteria (Part 1) , pp 1-17
- Publication Date: February 2024
- Language: English
Recent Advances in CRISPR-Cas Genome Engineering: An Overview, Page 1 of 1
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Bacteria is one of the most primitive organisms on earth. Its high susceptibility to bacteriophages has tailored them to use specific tools to edit their genome and evade the bacteriophages. This defense system has been developed to be the most specific genome editing technology of this current period. Previously, various other tools such as restriction enzymes (RE), zinc finger nucleases (ZNF), and transcription activator-like effector nucleases (TALENS) were utilized. Still, its major limitations led to exploiting the bacterial defense system to edit the genome. CRISPR technology can be applied in various microbiology, pathology, cancer biology, molecular biology, and industrial biotechnology, but its limitations, such as off-target effects due to unspecific alterations, are a major concern. In the future, this effective gene alteration technology will be developed to treat inherited rare genetic disorders. This chapter highlights the discovery, components, applications, limitations, and future prospects of CRISPR-Cas.
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