Overview and Applications of CRISPR/Cas9 Based Genome Editing in Industrial Microorganisms
- Authors: Kiran Dindhoria1, Vivek Manyapu2, Ashif Ali3, Aman Kumar4, Rakshak Kumar5
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View Affiliations Hide Affiliations1 Biotechnology Division, CSIR Institute of Himalayan Bioresource Technology Palampur, Himachal Pradesh-176061, India 2 Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology Palampur, Himachal Pradesh-176061, India 3 Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology Palampur, Himachal Pradesh-176061, India 4 Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology Palampur, Himachal Pradesh-176061, India 5 Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology Palampur, Himachal Pradesh-176061, India
- Source: Genome Editing in Bacteria (Part 1) , pp 18-44
- Publication Date: February 2024
- Language: English
Overview and Applications of CRISPR/Cas9 Based Genome Editing in Industrial Microorganisms, Page 1 of 1
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CRISPR-Cas technology has reshaped the field of microbiology. It has improved the microbial strains for better industrial and therapeutic utilization. In this chapter, we have tried to provide an overview of this technology with special reference to its associated applications in the various fields of interest. We have discussed the origin, classification, and different genome editing methods of CRISPR-Cas to understand its historical significance and the basic mechanism of action. Further, different applications in the area of agriculture, food industry, biotherapeutics, biofuel, and other valuable product synthesis were also explained to highlight the advancement of this system in industrial microbes. We have also tried to review some of the limitations offered by CRISPR and insights into its future perspective.
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