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image of Antidepressant Efficacy of Nutraceutical Gummies: Formulation of Withania Somnifera and Valeriana officinalis Evaluated in Force Swim Test Model

Abstract

Objective

This investigation aims to elucidate the potential of nutraceuticals as a therapeutic modality for depression by enhancing the solubility and bioavailability of (Ashwagandha) using specific solid dispersion techniques.

Methods

Solid Withania somnifera and Valeriana officinalis dispersions were prepared hot melt extrusion and solvent evaporation. These processes increased the aqueous solubility of Ashwagandha by 3.2-fold (from 44.2 µg/mL to 142 µg/mL). An optimal 1:2 drug-to-hydrotropic agent ratio was selected based on comprehensive DSC, PXRD, and FTIR characterisation, indicating partial amorphisation and effective molecular interactions with β-cyclodextrin. The resulting solid dispersion was incorporated into nutraceutical gummies, formulated using type B gelatin, high-methoxyl pectin, and food-grade guar gum a standardized molding process. The antidepressant efficacy of these gummies was assessed in murine models using the Forced Swim Test, with quantitative evaluation of changes in immobility time and climbing behavior.

Results

The solid dispersion methodology significantly enhanced Ashwagandha’s solubility (3.2-fold increase). In the Forced Swim Test, animals treated with the gummy formulation exhibited a 40% reduction in immobility time and a 35% increase in climbing activity compared to controls, indicating a robust antidepressant effect.

Conclusion

This study presents a novel nutraceutical approach to managing depression. By optimizing the solubility and bioavailability of key herbal constituents through advanced solid dispersion techniques, the gummy formulation offers a promising alternative or adjunctive treatment for depressive disorders.

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2025-06-11
2025-09-26

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Antidepressant Efficacy of Nutraceutical Gummies: Formulation of Withania Somnifera and Valeriana officinalis Evaluated in Force Swim Test Model
Bentham Science Publishers ; https://doi.org/10.2174/012772574X375447250528171314
/content/journals/rafna/10.2174/012772574X375447250528171314
/content/journals/rafna/10.2174/012772574X375447250528171314
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  • Article Type:     Research Article
Keywords: depression ; phytoconstituents ; valerian root ; forced swim test ; ashwagandha ; antidepressant therapy ; Nutraceuticals ; withania somnifera

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