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2000
Volume 16, Issue 3
  • ISSN: 2772-574X
  • E-ISSN: 2772-5758

Abstract

Introduction

L., commonly known as red goji berry, is a widely recognized plant-based medicinal herb with nutritional and therapeutic properties. In this study, the effects of various cytokinins on the germination of seeds and the growth of seedlings were investigated under conditions.

Methods

The berries were first surface sterilized and dissected, and the seeds were then cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (0.5, 1.0, and 1.5 mg/L) of 6-benzylaminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN) for 10 weeks at 25±2ºC with a photoperiod of 16 hours and a light intensity of 1000 lux. Upon observation after 10 weeks of culture, all cytokinin-treated cultures produced 100% seed germination as early as 7 days. KIN at 0.5 mg/L produced plantlets with the greatest height (8.40 ± 0.97 cm) with extensive rooting and the greatest total chlorophyll production. Besides, KIN at 1.5 mg/L resulted in the highest number of leaves per plantlet (6.90 ± 0.72), while 1.0 mg/L of TDZ led to the greatest biomass, i.e., fresh weight (FW) of 0.328 ± 0.05 gram and dry weight (DW) of 0.023 ± 0.003 gram.

Results

All cytokinins used in this experiment (BAP, TDZ, and KIN) promoted different growth promotion responses in . The effects of different types and concentrations of cytokinin on the height of plantlets, number of leaves per plantlet, fresh and dry weight, the extent of rooting, and the chlorophyll content were demonstrated to be statistically significant.

Conclusion

This study provides valuable insights into optimizing cultivation techniques for goji berry propagation, which could contribute to developing superior cultivars and increased production of this superfruit in the future. For future perspectives, extended research in elucidating the underlying mechanism associated with cytokinin supplementation is imperative in understanding the roles of cytokinins and optimizing their effects on plant growth promotion.

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2025-11-01
2025-11-16

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References

  1. TugumeP. NyakoojoC. Ethno-pharmacological survey of herbal remedies used in the treatment of paediatric diseases in Buhunga parish, Rukungiri District, Uganda.BMC Complement. Altern. Med.201919135310.1186/s12906‑019‑2763‑6 31806007
     [Google Scholar]
  2. DeheleanC.A. MarcoviciI. SoicaC. MiocM. CoricovacD. IurciucS. CretuO.M. PinzaruI. Plant-derived anticancer compounds as new perspectives in drug discovery and alternative therapy.Molecules2021264110910.3390/molecules26041109 33669817
     [Google Scholar]
  3. WenliS. ShahrajabianM.H. QiC. Health benefits of wolfberry (Gou Qi Zi, Fructus barbarum L.) on the basis of ancient Chineseherbalism and Western modern medicine.Avicenna J. Phytomed.2021112109119 33907670
     [Google Scholar]
  4. MaZ.F. ZhangH. TehS.S. WangC.W. ZhangY. HayfordF. Goji berries as a potential natural antioxidant medicine: an insight into their molecular mechanisms of action.Oxid. Med. Cell. Longev.20192437397
     [Google Scholar]
  5. VidovićB.B. MilinčićD.D. MarčetićM.D. DjurišJ.D. IlićT.D. KostićA. Health benefits and applications of goji berries in functional food products development: A review.Antioxidants2022112248
     [Google Scholar]
  6. ChenJ. ChaoC.T. WeiX. Gojiberry breeding: Current status and future prospects.In: Breeding and Health Benefits of Fruit and Nut Crops.InTech2018
     [Google Scholar]
  7. BridgenM.P. Van HoutvenW. EeckhautT. Plant tissue culture techniques for breeding.Ornamental Crops. Handbook of Plant Breeding2018Springer: Cham1112714410.1007/978‑3‑319‑90698‑0_6
     [Google Scholar]
  8. FarhanaN. Seed biology and phytochemistry for sustainable future.Seed Biology Updates.IntechOpen2022
     [Google Scholar]
  9. BishtT.S. RawatL. ChakrabortyB. YadavV. A recent advances in use of plant growth regulators (PGRs) in fruit crops - A review.Int. J. Curr. Microbiol. Appl. Sci.2018751307133610.20546/ijcmas.2018.705.159
     [Google Scholar]
  10. AkhtarS.S. MekureyawM.F. PandeyC. RoitschT. Role of cytokinins for interactions of plants with microbial pathogens and pest insects.Front. Plant Sci.2020101777
     [Google Scholar]
  11. AkramM. AftabF. Effect of cytokinins on in vitro seed germination and changes in chlorophyll and soluble protein contents of teak (Tectona grandis L.).Biochem. Physiol.201543166
     [Google Scholar]
  12. LichtenthalerH.K. WellburnA.R. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents.Biochem. Soc. Trans.198311559159210.1042/bst0110591
     [Google Scholar]
  13. AnetaS. Effects of different cytokinins on chlorophyll retention in the moss Bryum argenteum.Periodicum Biologorum20101123301305
     [Google Scholar]
  14. SharmaS. KaurP. GaikwadK. Role of cytokinins in seed development in pulses and oilseed crops: Current status and future perspective.Front. Genet.202213940660
     [Google Scholar]
  15. GuanC. WangX. FengJ. HongS. LiangY. RenB. ZuoJ. Cytokinin antagonizes abscisic acid-mediated inhibition of cotyledon greening by promoting the degradation of abscisic acid insensitive5 protein in Arabidopsis.Plant Physiol.201416431515152610.1104/pp.113.234740 24443524
     [Google Scholar]
  16. NikolićR. MitićN. MiletićR. NeškovićM. Effects of cytokinins on in vitro seed germination and early seedling morphogenesis in Lotus corniculatus L.J. Plant Growth Regul.200625318719410.1007/s00344‑005‑0129‑4
     [Google Scholar]
  17. Al-KhafajiZ.H. Al-BurkiF.R. Study of the effect of salt stress and kinetin and their interaction on the growth and yield of wheat (Triticum aestivum L.).IOP Conf. Ser. Earth Environ. Sci.2021923101208410.1088/1755‑1315/923/1/012084
     [Google Scholar]
  18. WuW. DuK. KangX. WeiH. The diverse roles of cytokinins in regulating leaf development.Hortic. Res.20218118
     [Google Scholar]
  19. WernerT. Regulation of plant growth by cytokinin.Proc. Natl. Acad. Sci. USA200198181048710492
     [Google Scholar]
  20. HuangW. RatkowskyD.A. HuiC. WangP. SuJ. ShiP. Leaf fresh weight versus dry weight: Which is better for describing the scaling relationship between leaf biomass and leaf area for broad-leaved plants?Forests201910325610.3390/f10030256
     [Google Scholar]
  21. SchallerG.E. StreetI.H. KieberJ.J. Cytokinin and the cell cycle.Curr. Opin. Plant Biol.20142171510.1016/j.pbi.2014.05.015
     [Google Scholar]
  22. PrasadR. Cytokinin and its key role to enrich the plant nutrients and growth under adverse conditions-an update.Front. Genet.202213883924
     [Google Scholar]
  23. GitelsonA.A. GritzY. MerzlyakM.N. Relationships between leaf chlorophyll content and spectral reflectance and algorithms for non-destructive chlorophyll assessment in higher plant leaves.J. Plant Physiol.2003160
     [Google Scholar]
  24. LiY. HeN. HouJ. XuL. LiuC. ZhangJ. WangQ. ZhangX. WuX. Factors influencing leaf chlorophyll content in natural forests at the biome scale.Front. Ecol. Evol.20186JUN6410.3389/fevo.2018.00064
     [Google Scholar]
  25. KaulK. SabharwalP.S. Effects of sucrose and kinetin on growth and chlorophyll synthesis in tobacco tissue cultures.Plant Physiol.197147.
     [Google Scholar]
  26. Pazurkiewicz-KocotK. KitaA. HaduchA. The effect of kinetin on the chlorophyll pigments content in the leaves of Zea mays L. seedlings and accumulation of some metal ions.Eng. Environ. Protect.2011144397409
     [Google Scholar]
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Growth Promotion and In Vitro Seed Germination of Lycium barbarum L. (Red Goji) Using Different Types of Cytokinins
Recent Pat Food Nutr Agric 16, 334 (2025); https://doi.org/10.2174/012772574X313421240902052600
/content/journals/rafna/10.2174/012772574X313421240902052600
/content/journals/rafna/10.2174/012772574X313421240902052600
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  • Article Type:     Research Article
Keyword(s): 6-benzylaminopurine; chlorophyll; in vitro germination; kinetin; Red goji; thidiazuron

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