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2000
Volume 21, Issue 1
  • ISSN: 2772-4344
  • E-ISSN: 2772-4352

Abstract

Introduction

Nanoparticles obtained through green synthesis play remarkable roles in biomedical applications. Urinary tract infections (UTIs) are a nightmare for the mass population, especially for women, and quinolone-resistant UTI bacteria worsen the situation. Our current investigation aimed to control quinolone-resistant pathogenic UTI bacteria with green-synthesized silver nanoparticles (AgNPs).

Methods

Visual observation of color change, UV-Vis spectroscopic analysis, FTIR (Fourier Transform Infrared Spectroscopy), DLS (Dynamic Light Scattering), XRD (X-ray Diffraction), and TEM (Transmission Electron Microscopy) techniques were used to effectively characterize the biosynthesized AgNPs. sp., and bacteria were isolated and identified using biochemical and molecular identification techniques from urine samples of hospitalized patients with UTI. These bacteria showed quinolone resistance to up to fourth-generation antibiotics.

Results and Discussion

The results elucidated the synthesis of spherical-shaped nano-silvers coated with polyphenols. These biosynthesized AgNPs showed moderate polydispersity and narrow distribution. The antibacterial efficiency of the AgNPs was determined against isolated bacterial strains. and exhibited the highest sensitivity to the nanoparticles. Nanoparticles at a concentration of 128 µg/ml inhibited bacterial growth to a great extent and gave a maximum inhibition zone of 14.67 ± 0.577 mm in diameter for both bacterial strains. In addition, toxicity analysis of synthesized nanoparticles brine shrimp lethality assay (BSLA) showed a very low cytotoxicity level (2398.83 µg/ml), depicting safety for human use.

Conclusion

We can conclude that leaf-synthesized AgNPs could possess significant biomedical applications as potential antibacterial agents due to their bactericidal activity and low cytotoxicity.

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2026-02-28

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References

  1. AhsanA. FarooqM.A. AhsanB.A. ParveenA. Green synthesis of silver nanoparticles using Parthenium hysterophorus: Optimization, characterization and in vitro therapeutic evaluation.Molecules20202515332410.3390/molecules25153324 32707950
     [Google Scholar]
  2. MelkamuW.W. BitewL.T. Green synthesis of silver nanoparticles using Hagenia abyssinica (Bruce) J.F. Gmel plant leaf extract and their antibacterial and anti-oxidant activities.Heliyon2021711e0845910.1016/j.heliyon.2021.e08459 34901505
     [Google Scholar]
  3. AsifM. YasminR. AsifR. AmbreenA. MustafaM. UmbreenS. Green synthesis of silver nanoparticles (AgNPs), structural characterization, and their antibacterial potential.Dose Response20222021559325822108870910.1177/15593258221088709 35592270
     [Google Scholar]
  4. CapuzzoA. Bacterial synthesis of nanoparticles: Current trends in biotechnology and biomedical fields.Ann Adv Biomed Sci202111810.23880/aabsc‑16000161
     [Google Scholar]
  5. BaydaS. AdeelM. TuccinardiT. CordaniM. RizzolioF. The history of nanoscience and nanotechnology: From chemical–physical applicati-ons to nanomedicine.Molecules201925111210.3390/molecules25010112 31892180
     [Google Scholar]
  6. KanchanaR. FernandesA. Biogenesis of silver nanoparticles from agro-waste.Asian J. Pharm. Clin. Res.2019415357
     [Google Scholar]
  7. EdisonT.J.I. SethuramanM.G. Biogenic robust synthesis of silver nanoparticles using Punica granatum peel and its application as a green catalyst for the reduction of an anthropogenic pollutant 4-nitrophenol.Spectrochim. Acta A Mol. Biomol. Spectrosc.201310426226410.1016/j.saa.2012.11.084 23274256
     [Google Scholar]
  8. SerdarG. Green biosynthesis of silver nanoparticles were obtained from the extract of pomegranate (Punica granatum L.) leaves by supercritical extraction using microwave method.Celal Bayar Univ J Sci20231935810.18466/cbayarfbe.1338606
     [Google Scholar]
  9. MaphetuN. UnuofinJ.O. MasukuN.P. OlisahC. LebeloS.L. Medicinal uses, pharmacological activities, phytochemistry, and the molecular mechanisms of Punica granatum L. (pomegranate) plant extracts: A review.Biomed. Pharmacother.202215311325610.1016/j.biopha.2022.113256 36076615
     [Google Scholar]
  10. KhanN. KhanD. RahmanI. UllahF. NisarM. AliK. Ethnobotanical and ecological study of Punica granatum in Dir district, Khyber Pakhtunkhwa, Pakistan.Regul. Mech. Biosyst.201884652661
     [Google Scholar]
  11. JainA.S. PawarP.S. SarkarA. JunnuthulaV. DyawanapellyS. Bionanofactories for green synthesis of silver nanoparticles: Toward antimicrobial applications.Int. J. Mol. Sci.202122211199310.3390/ijms222111993 34769419
     [Google Scholar]
  12. CaoD. ShenY. HuangY. Levofloxacin versus ciprofloxacin in the treatment of urinary tract infections: Evidence-based analysis.Front. Pharmacol.20211265809510.3389/fphar.2021.658095 33897441
     [Google Scholar]
  13. SimõesESAC OliveiraEA MakRH Urinary tract infection in pediatrics: An overview.J Pediatr202096(Suppl 1)10.1016/j.jped.2019.10.006
     [Google Scholar]
  14. WangR. LaSalaC. Role of antibiotic resistance in urinary tract infection management: a cost-effectiveness analysis.Am. J. Obstet. Gynecol.20212255550.e1550.e1010.1016/j.ajog.2021.08.014 34418350
     [Google Scholar]
  15. FoxmanB. The epidemiology of urinary tract infection.Nat. Rev. Urol.201071265366010.1038/nrurol.2010.190 21139641
     [Google Scholar]
  16. NeugentM.L. HulyalkarN.V. NguyenV.H. ZimmernP.E. De NiscoN.J. Advances in understanding the human urinary microbiome and its potential role in urinary tract infection.MBio2020112e00218e0022010.1128/mBio.00218‑20 32345639
     [Google Scholar]
  17. OlinS.J. BartgesJ.W. Urinary tract infections treatment/comparative therapeutics.Vet. Clin. North Am. Small Anim. Pract.202252358160810.1016/j.cvsm.2022.01.002 35465902
     [Google Scholar]
  18. HutchingsM.I. TrumanA.W. WilkinsonB. Antibiotics: past, present and future.Curr. Opin. Microbiol.201951728010.1016/j.mib.2019.10.008 31733401
     [Google Scholar]
  19. FrieriM. KumarK. BoutinA. Antibiotic resistance.J. Infect. Public Health201710436937810.1016/j.jiph.2016.08.007 27616769
     [Google Scholar]
  20. KangX. YanJ. HuangF. YangL. On the mechanism of antibiotic resistance and fecal microbiota transplantation.Math. Biosci. Eng.20191667057708410.3934/mbe.2019354 31698603
     [Google Scholar]
  21. DunneM.W. AroninS.I. DasA.F. Sulopenem or ciprofloxacin for the treatment of uncomplicated urinary tract infections in women: A phase 3, randomized trial.Clin. Infect. Dis.2023761667710.1093/cid/ciac738 36069202
     [Google Scholar]
  22. NaeemA. BadshahS. MuskaM. AhmadN. KhanK. The Current case of quinolones: Synthetic approaches and antibacterial activity.Molecules201621426810.3390/molecules21040268 27043501
     [Google Scholar]
  23. AtnkutB. NigussieA. GebreamanuleB. KumeraB. AstatkieT. Assessment of inappropriate use of antibiotics and contributing factors in Awi Administrative Zone, Northwestern Amhara regional State, Ethiopia.New Microbes New Infect.20256310155710.1016/j.nmni.2024.101557 39807160
     [Google Scholar]
  24. SunD. JeannotK. XiaoY. KnappC.W. Horizontal gene transfer mediated bacterial antibiotic resistance.Front. Microbiol.20191010.3389/fmicb.2019.01933
     [Google Scholar]
  25. C Reygaert W.An overview of the antimicrobial resistance mechanisms of bacteria.AIMS Microbiol.20184348250110.3934/microbiol.2018.3.482 31294229
     [Google Scholar]
  26. BonnaA.S. PavelS.R. FerdousJ. KhanS.A. AliM. Antibiotic resistance: An increasingly threatening but neglected public health challenge in Bangladesh.Int J Surg Open20224910058110.1016/j.ijso.2022.100581
     [Google Scholar]
  27. MuteebG. RehmanM.T. ShahwanM. AatifM. Origin of antibiotics and antibiotic resistance, and their impacts on drug development: A narrative review.Pharmaceuticals20231611161510.3390/ph16111615 38004480
     [Google Scholar]
  28. MoradiF. GhaediA. FooladfarZ. BazrgarA. Recent advance on nanoparticles or nanomaterials with anti-multidrug resistant bacteria and anti-bacterial biofilm properties: A systematic review.Heliyon2023911e2210510.1016/j.heliyon.2023.e22105 38034786
     [Google Scholar]
  29. MoradiF. AkbariM. Vakili-GhartavolR. OstovariM. HadiN. Molecular characterization of superbugs K. pneumoniae harboring extended-spectrum β-lactamase (ESBL) and carbapenemase resistance genes among hospitalized patients in southwestern Iran, Western Asia.Heliyon20241017e3685810.1016/j.heliyon.2024.e36858 39263100
     [Google Scholar]
  30. SalamM.A. Al-AminM.Y. SalamM.T. Antimicrobial resistance: A growing serious threat for global public health.Health care20231113194610.3390/healthcare11131946
     [Google Scholar]
  31. BahramiM. SeratiS.P. MoradiF. HadiN. sabbaghi N, Eslaminezhad S. How nanomaterials act against bacterial structures? a narrative review focusing on nanoparticle molecular mechanisms.Microb. Pathog.202419610700210.1016/j.micpath.2024.107002 39393474
     [Google Scholar]
  32. YinI.X. ZhangJ. ZhaoI.S. MeiM.L. LiQ. ChuC.H. The antibacterial mechanism of silver nanoparticles and its application in dentistry.Int. J. Nanomed.2020152555256210.2147/IJN.S246764 32368040
     [Google Scholar]
  33. BrunaT. Maldonado-BravoF. JaraP. CaroN. Silver nanoparticles and their antibacterial applications.Int. J. Mol. Sci.20212213720210.3390/ijms22137202 34281254
     [Google Scholar]
  34. JamalM.A. UddinH. RahmanS. Biogenic synthesis of Silver Nanoparticles using Azadirachta indica (Neem) leaf extract targeting inhibitory action against antibiotic resistance bacteria.Antiinfect. Agents202119510.2174/2211352519666210120085253
     [Google Scholar]
  35. Al MashudM.A. MoinuzzamanM. HossainM.S. Green synthesis of silver nanoparticles using Cinnamomum tamala (Tejpata) leaf and their potential application to control multidrug resistant Pseudomonas aeruginosa isolated from hospital drainage water.Heliyon202287e0992010.1016/j.heliyon.2022.e09920 35855998
     [Google Scholar]
  36. JalabJ. AbdelwahedW. KitazA. Al-KayaliR. Green synthesis of silver nanoparticles using aqueous extract of Acacia cyanophylla and its antibacterial activity.Heliyon202179e0803310.1016/j.heliyon.2021.e08033 34611564
     [Google Scholar]
  37. KhaneY. BenouisK. AlbukhatyS. Green synthesis of silver nanoparticles using aqueous Citrus limon zest extract: Characterization and evaluation of their antioxidant and antimicrobial properties.Nanomaterials20221212201310.3390/nano12122013 35745352
     [Google Scholar]
  38. WidatallaH.A. YassinL.F. AlrasheidA.A. Green synthesis of silver nanoparticles using green tea leaf extract, characterization and evaluation of antimicrobial activity.Nanoscale Adv.20224391191510.1039/D1NA00509J 36131825
     [Google Scholar]
  39. ReddyN.V. LiH. HouT. BethuM.S. RenZ. ZhangZ. Phytosynthesis of silver nanoparticles using Perilla frutescens leaf extract: Characterization and evaluation of antibacterial, antioxidant, and anticancer activities.Int. J. Nanomed.202116152910.2147/IJN.S265003 33447027
     [Google Scholar]
  40. TascaF. AntiochiaR. Biocide activity of green quercetin-mediated synthesized silver nanoparticles.Nanomaterials202010590910.3390/nano10050909 32397267
     [Google Scholar]
  41. HabeebR.H.B. DhandapaniR. NarayananS. Medicinal plants mediated the green synthesis of silver nanoparticles and their biomedical applications.IET Nanobiotechnol.202216411514410.1049/nbt2.12078 35426251
     [Google Scholar]
  42. EzeadorCO EjikeugwuPC UshieSN AgbakobaNR Isolation, identification and prevalence of pseudomonas aeruginosa isolates from clinical and environmental sources in Onitsha Metropolis, Anambra State.Eu J Med Health Sci20202210.24018/ejmed.2020.2.2.188
     [Google Scholar]
  43. JamilS. KhanR.A. AfrozS. AhmedS. Phytochemistry, Brine shrimp lethality and mice acute oral toxicity studies on seed extracts of Vernonia anthelmintica.Pak. J. Pharm. Sci.201629620532057 28375123
     [Google Scholar]
  44. WaghuldeS. KaleM.K. PatilV. Brine shrimp lethality assay of the aqueous and ethanolic extracts of the selected species of medicinal plants.Proceedings201941147
     [Google Scholar]
  45. ThangaveluS. DhandapaniR. ArulprakasamA. Isolation, identification, characterization, and plasmid profile of urinary tract infectious Escherichia coli from clinical samples.Evid. Based Complement. Alternat. Med.20222022723458610.1155/2022/7234586 35356239
     [Google Scholar]
  46. AdhikariB. KwonY.M. Cell density alters bacterial community structure in culture-enriched 16S rRNA gene microbiota profiling.BMC Res. Notes202013126910.1186/s13104‑020‑05113‑2 32493423
     [Google Scholar]
  47. YaoH. LiuJ. JiangX. ChenF. LuX. ZhangJ. Analysis of the clinical effect of combined drug susceptibility to guide medication for carbapenem-resistant Klebsiella pneumoniae patients based on the kirby–bauer disk diffusion method.Infect. Drug Resist.202114798710.2147/IDR.S282386 33469322
     [Google Scholar]
  48. WeinsteinM.P. LewisJ.S. The clinical and laboratory standards Institute subcommittee on antimicrobial susceptibility testing: Background, organization, functions, and processes.J. Clin. Microbiol.2020583e01864e1910.1128/JCM.01864‑19 31915289
     [Google Scholar]
  49. DashC. J Payyappilli R. KOH string and Vancomycin susceptibility test as an alternative method to Gram staining.J Int Med Dent201632889010.18320/JIMD/201603.0288
     [Google Scholar]
  50. ChavanD KhatoonH AnokheA KaliaV. Oxidase test: A biochemical method in bacterial identification.AgriCos e-Newslett2022331-3
     [Google Scholar]
  51. DiwakarM.K. GoyalA. GoyalS. Resistance pattern of methicillin resistant Staphylococcus aureus among nasal isolates of HIV infected patients in a tertiary care hospital.Int J Res Med Sci20197242843310.18203/2320‑6012.ijrms20190347
     [Google Scholar]
  52. BhutiaM.O. ThapaN. TamangJ.P. Molecular Characterization of bacteria, detection of enterotoxin genes, and screening of antibiotic susceptibility patterns in traditionally processed meat products of Sikkim, India.Front. Microbiol.20211159960610.3389/fmicb.2020.599606 33505372
     [Google Scholar]
  53. IslamF. RoyN. Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses.BMC Res. Notes201811144510.1186/s13104‑018‑3558‑4 29973263
     [Google Scholar]
  54. HallB.G. Building phylogenetic trees from molecular data with MEGA.Mol. Biol. Evol.20133051229123510.1093/molbev/mst012 23486614
     [Google Scholar]
  55. VazourasK. VelaliK. TassiouI. Antibiotic treatment and antimicrobial resistance in children with urinary tract infections.J. Glob. Antimicrob. Resist.20202041010.1016/j.jgar.2019.06.016 31252156
     [Google Scholar]
  56. IrfanM. MunirH. IsmailH. Moringa oleifera gum based silver and zinc oxide nanoparticles: green synthesis, characterization and their antibacterial potential against MRSA.Biomater. Res.20212511710.1186/s40824‑021‑00219‑5 33964968
     [Google Scholar]
  57. ShameliK. AhmadM.B. JazayeriS.D. Investigation of antibacterial properties silver nanoparticles prepared via green method.Chem. Cent. J.2012617310.1186/1752‑153X‑6‑73 22839208
     [Google Scholar]
  58. RafiqueM. SadafI. TahirM.B. Novel and facile synthesis of silver nanoparticles using Albizia procera leaf extract for dye degradation and antibacterial applications.Mater. Sci. Eng. C2019991313132410.1016/j.msec.2019.02.059 30889666
     [Google Scholar]
  59. NiluxsshunM.C.D. MasilamaniK. MathiventhanU. Green synthesis of silver nanoparticles from the extracts of fruit peel of citrus tangerina, Citrus sinensis, and citrus limon for antibacterial activities.Bioinorg. Chem. Appl.202120211810.1155/2021/6695734 33623527
     [Google Scholar]
  60. BhatM. ChakrabortyB. KumarR.S. Biogenic synthesis, characterization and antimicrobial activity of Ixora brachypoda (DC) leaf extract mediated silver nanoparticles.J. King Saud Univ. Sci.202133210129610.1016/j.jksus.2020.101296
     [Google Scholar]
  61. LiP.J. PanJ.J. TaoL.J. Green synthesis of silver nanoparticles by extracellular extracts from Aspergillus japonicus PJ01.Molecules20212615447910.3390/molecules26154479 34361632
     [Google Scholar]
  62. AygünA. ÖzdemirS. GülcanM. YalçınM.S. UçarM. ŞenF. Characterization and antioxidant-antimicrobial activity of silver nanoparticles synthesized using Punica granatum extract.Int. J. Environ. Sci. Technol.20221942781278810.1007/s13762‑021‑03246‑w
     [Google Scholar]
  63. PungleR. NileS.H. MakwanaN. SinghR. SinghR.P. KharatA.S. Green synthesis of silver nanoparticles using the Tridax procumbens plant extract and screening of its antimicrobial and anticancer activities.Oxid. Med. Cell. Longev.2022202211410.1155/2022/9671594 35795854
     [Google Scholar]
  64. SwilamN. NematallahK.A. Polyphenols profile of pomegranate leaves and their role in green synthesis of silver nanoparticles.Sci. Rep.20201011485110.1038/s41598‑020‑71847‑5 32908245
     [Google Scholar]
  65. KumarM. RanjanR. SinhaM. Impact of aqueous leaf extract of Punica granatum and synthesized silver nanoparticles against streptozotocin induced diabetes in rats.SSRN202410.5772/intechopen.1003780
     [Google Scholar]
  66. AksonoE.B. LatifahA.C. SuwantiL.T. HaqK.U. PertiwiH. Clove flower extract (Syzygium aromaticum) has anticancer potential effect analyzed by molecular docking and brine shrimp lethality test (BSLT).Vet. Med. Int.202220221710.1155/2022/5113742 36106174
     [Google Scholar]
  67. Rodríguez-MedinaN. Barrios-CamachoH. Duran-BedollaJ. Garza-RamosU. Klebsiella variicola: An emerging pathogen in humans.Emerg. Microbes Infect.20198197398810.1080/22221751.2019.1634981 31259664
     [Google Scholar]
  68. PangB. YuH. ZhangJ. YeF. WuH. ShangC. Identification of differentially expressed genes for Pseudomonas sp. Cr13 stimulated by hexavalent chromium.PLoS One2022178e027252810.1371/journal.pone.0272528 35930609
     [Google Scholar]
  69. UllC. YilmazE. BaeckerH. SchildhauerT. WaydhasC. HamsenU. Microbial findings and the role of difficult-to-treat pathogens in patients with periprosthetic infection admitted to the intensive care unit.Orthop. Rev.2020123886710.4081/or.2020.8867 33312492
     [Google Scholar]
  70. PeriakaruppanR. PraveenaM.S. PriyaC. RanjithaP. RajG.S. DanarajJ. Biosynthesis of silica nanoparticles using the leaf extract of Punica granatum and assessment of its antibacterial activities against human pathogens.Appl. Biochem. Biotechnol.2022194115594560510.1007/s12010‑022‑03994‑6 35679016
     [Google Scholar]
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Green Synthesis of Silver Nanoparticles using Punica granatum Leaf Extract: A Novel Approach to Combat Quinolone-Resistant Urinary Tract Infective Pathogens
Recent Adv Antiinfect Drug Discov 21, 59 (2026); https://doi.org/10.2174/0127724344386982250826062715
/content/journals/raaidd/10.2174/0127724344386982250826062715
/content/journals/raaidd/10.2174/0127724344386982250826062715
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  • Article Type:     Research Article
Keyword(s): antibacterial agents; drug-resistance; Nanoparticles; Punica granatum; quinolones; urinary tract infections

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