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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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D-Serine-Dehydratase from Saccaromyces cerevisiae: A Pyridoxal 5'- phosphate-Dependent Enzyme for Advanced Biotech Applications
- Source: Protein and Peptide Letters, Volume 19, Issue 6, Jun 2012, p. 592 - 595
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- 01 Jun 2012
Abstract
The Saccaromices cerevisiae D-serine dehydratase is a pyridoxal 5'-phosphate dependent enzyme that requires zinc for its function. It catalyses the conversion of D-serine into pyruvate and ammonia with the K(m) and k(cat) values of 0.39 mM and 13.1 s(-1) respectively. In this work, a new methodology for monitoring D-serine is presented. Our results show that this enzyme could be successfully used as a biological probe for detection of D-serine via fluorescence spectroscopy.
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