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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Purification and Characterization of Human Brain Serine Racemase Expressed in Moderately Halophilic Bacteria
- Source: Protein and Peptide Letters, Volume 16, Issue 2, Feb 2009, p. 201 - 206
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- 01 Feb 2009
Abstract
We have successfully expressed an active human brain serine racemase (hSR) with His-tag using moderate halophile. The purified His-hSR showed high elimination and racemization activities on L-serine: the elimination activity was 2.6-fold higher than racemization activity. Both enzyme activities showed an optimum reaction pH at around 9.0 and were stimulated 5- to 7-fold by such divalent cations as Mg++, Mn++ and Ca++.
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