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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Cloning, Expression, Purification, Characterization, Crystallization and X-Ray Diffraction of Bifunctional Pyrimidine Deaminase/Reductase from Shigella flexneri 2a
- Source: Protein and Peptide Letters, Volume 14, Issue 9, Sep 2007, p. 925 - 927
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- 01 Sep 2007
Abstract
Bifunctional pyrimidine deaminase/reductase (RibD) plays an important role during riboflavin biosynthesis in many microorganisms. The 40.4 kDa RibD from Shigella flexneri 2a has been cloned, expressed, purified and characterized. Three Crystals of RibD have been obtained by the hanging-drop technique at 291 K using PEG 20k or NaCl as precipitant. The RibD crystal using PEG 20k as precipitant diffracted to 2.5Å.
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