Crystallization and Preliminary X-Ray Analysis of the Catalytic Domain of Chitinase D from Bacillus circulans WL-12

Yuichiro Kezuka; Kenji Bando; Hajime Kobayashi; Yasuo Yonou; Toshiya Sato; Takeshi Watanabe; Takamasa Nonaka

doi:10.2174/092986606778256108

ISSN: 0929-8665
E-ISSN: 1875-5305

Crystallization and Preliminary X-Ray Analysis of the Catalytic Domain of Chitinase D from Bacillus circulans WL-12
Authors: Yuichiro Kezuka¹, Kenji Bando², Hajime Kobayashi³, Yasuo Yonou⁴, Toshiya Sato⁵, Takeshi Watanabe⁶ and Takamasa Nonaka⁷
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¹ Department of BioEngineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata 940-2188, Japan. ² Department of BioEngineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata 940-2188, Japan. ³ Department of BioEngineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata 940-2188, Japan. ⁴ Department of BioEngineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata 940-2188, Japan. ⁵ Department of BioEngineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata 940-2188, Japan. ⁶ Department of BioEngineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata 940-2188, Japan. ⁷ Department of BioEngineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata 940-2188, Japan.
Source: Protein and Peptide Letters, Volume 13, Issue 9, Sep 2006, p. 951 - 954
DOI: https://doi.org/10.2174/092986606778256108
- Available online: 01 Sep 2006

Abstract

We report here on crystallization and preliminary X-ray analysis of the catalytic domain of chitinase D from Bacillus circulans WL-12. The native crystals of this domain were found to belong to the orthorhombic space group P212121. To elucidate the structure of the catalytic domain by the multiple isomorphous replacement method, 30 kinds of derivatized crystals were prepared by soaking the native crystals into a mother liquor containing salts of heavy metal atoms. Difference Patterson maps calculated for four derivatives showed strong peaks in the Harker sections.

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2006-09-01

2025-09-02

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Article Type: Research Article

Keyword(s): Bacillus circulans WL-12; catalytic domain; chitinase D; crystallization; preliminary X-ray analysis

Crystallization and Preliminary X-Ray Analysis of the Catalytic Domain of Chitinase D from Bacillus circulans WL-12

Abstract

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