Preliminary X-Ray Crystallographic Analysis of Centrin from Ciliate Euplotes octocarinatus

Xiao-Jing He; Liu Li; Hai Hou; Yaqin Zhao; Aihua Liang; Zihe Rao

doi:10.2174/0929866054395842

ISSN: 0929-8665
E-ISSN: 1875-5305

Preliminary X-Ray Crystallographic Analysis of Centrin from Ciliate Euplotes octocarinatus
Authors: Xiao-Jing He¹, Liu Li², Hai Hou³, Yaqin Zhao⁴, Aihua Liang⁵ and Zihe Rao⁶
View Affiliations Hide Affiliations

¹ Laboratory of Structural Biology, Tsinghua University, Beijing, 100084, China & National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, China. ² Laboratory of Structural Biology, Tsinghua University, Beijing, 100084, China & National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, China. ³ Laboratory of Structural Biology, Tsinghua University, Beijing, 100084, China & National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, China. ⁴ Laboratory of Structural Biology, Tsinghua University, Beijing, 100084, China & National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, China. ⁵ Laboratory of Structural Biology, Tsinghua University, Beijing, 100084, China & National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, China. ⁶ Laboratory of Structural Biology, Tsinghua University, Beijing, 100084, China & National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, China.
Source: Protein and Peptide Letters, Volume 12, Issue 6, Aug 2005, p. 609 - 611
DOI: https://doi.org/10.2174/0929866054395842
- Available online: 01 Aug 2005

Abstract

Centrins are four-EF-hand Ca2+-binding proteins, which belong to the CaM super family. The centrin from ciliate Euplotes Octocarinatus has been expressed in Escherichia coli, purified and crystallized using the hanging-drop method. Rod-like crystals were grown and diffracted to 2.0 Å. The crystals belong to space group P222 and the unit-cell parameters are a=34.442 Å, b=48.954 Å, c=72.583 Å.

Article metrics loading...

/content/journals/ppl/10.2174/0929866054395842

2005-08-01

2025-09-02

From This Site

/content/journals/ppl/10.2174/0929866054395842

dcterms_title,dcterms_subject,pub_keyword

-contentType:Contributor -contentType:Concept -contentType:Institution

10

5

Full text loading...

/content/journals/ppl/10.2174/0929866054395842

Article Type: Review Article

Keyword(s): centrin; crystallization; preliminary x-ray studies

Preliminary X-Ray Crystallographic Analysis of Centrin from Ciliate Euplotes octocarinatus

Abstract

From This Site

Most Read This Month

Most Cited Most Cited RSS feed

The LL-37 Antimicrobial Peptide as a Treatment for Systematic Infection of Acinetobacter baumannii in a Mouse Model

Wogonin Restrains the Malignant Progression of Lung Cancer Through Modulating MMP1 and PI3K/AKT Signaling Pathway

FN1 Promotes Thyroid Carcinoma Cell Proliferation and Metastasis by Activating the NF-b Pathway

A Temporin Derived Peptide Showing Antibacterial and Antibiofilm Activities against Staphylococcus aureus

Upregulation of Connexins in the Rat Hippocampal and Cortical Neurons Following Blockade of NMDA Receptors During Postnatal Development

Long Non-coding RNA LINC00473 Promotes Breast Cancer Progression via miR-424-5p/CCNE1 Pathway

Protease Inhibitors (PIs): Candidate Molecules for Crop Protection Formulations against Necrotrophs

Preparation of IgE Antibody and Distribution of IgE⁺ Secretory Cells in the Palatine Tonsil of Bactrian Camel

A Functional Human Glycogen Debranching Enzyme Encoded by a Synthetic Gene: Its Implications for Glycogen Storage Disease Type III Management

The GA-Hecate Peptide inhibits the ZIKV Replicative Cycle in Different Steps and can Inhibit the Flavivirus NS2B-NS3 Protease after Cell Infection