Crystallization and Preliminary X-ray Analysis of Plant Class I Chitinase from Rice

Yuichiro Kezuka; Keiji Kitazaki; Yoshikane Itoh; Jun Watanabe; Osamu Takaha; Takeshi Watanabe; Yoko Nishizawa; Takamasa Nonaka

doi:10.2174/0929866043406968

ISSN: 0929-8665
E-ISSN: 1875-5305

Crystallization and Preliminary X-ray Analysis of Plant Class I Chitinase from Rice
Authors: Yuichiro Kezuka¹, Keiji Kitazaki², Yoshikane Itoh³, Jun Watanabe⁴, Osamu Takaha⁵, Takeshi Watanabe⁶, Yoko Nishizawa⁷ and Takamasa Nonaka⁸
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¹ Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, JAPAN ² Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, JAPAN ³ Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, JAPAN ⁴ Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, JAPAN ⁵ Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, JAPAN ⁶ Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, JAPAN ⁷ Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, JAPAN ⁸ Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, JAPAN
Source: Protein and Peptide Letters, Volume 11, Issue 4, Aug 2004, p. 401 - 405
DOI: https://doi.org/10.2174/0929866043406968
- Available online: 01 Aug 2004

Abstract

We report here on crystallization and preliminary X-ray analysis of plant class I chitinase from rice (OsChia1b). Similar single crystals of full-length OsChia1b were obtained under two independent conditions. The crystals grown under these conditions diffracted up to 2.1 and 2.5 Å resolution, respectively, at a synchrotron beamline, and were found to belong to the tetragonal space group P43212.

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2004-08-01

2025-10-09

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Article Type: Review Article

Keyword(s): class I chitinase; crystallization; elastase; preliminary x-ray analysis; protease inhibitor complex; rice (oryza sativa l. japonica); specificity

Crystallization and Preliminary X-ray Analysis of Plant Class I Chitinase from Rice

Abstract

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