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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Two-dimensional Protein Analysis by Isoelectric Focusing in a Multi-cell Plate System
- Source: Protein and Peptide Letters, Volume 9, Issue 1, Feb 2002, p. 39 - 43
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- 01 Feb 2002
Abstract
A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.
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