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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Production, Purification, and Biological Activity Analysis of Recombinant Human Persephin Expressed in Insect Cells
- Source: Protein and Peptide Letters, Volume 8, Issue 2, Apr 2001, p. 131 - 138
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- 01 Apr 2001
Abstract
Recombinant human Persephin (PSP) has been expressed at high levels in recombinant baculavirus infected Trichoplusia ni (Tn-5B1-4) cells. The expressed protein was purified by nickel affinity chromatography. Pure, recombinant human PSP promoted the survival of embryonic motor neurons in vitro but failed to protect adult rat spinal cord motor neurons after sciatic nerve transection in vivo. Because recombinant bioactive human PSP can be obtained in large quantities, and purified to near homogeneity, they are suitable for further biological activity investigation.
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