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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Functional Expression of a Galactose-Binding C-Type Lectin In Pichia pastoris
- Source: Protein and Peptide Letters, Volume 8, Issue 3, Jun 2001, p. 217 - 224
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- 01 Jun 2001
Abstract
Trimeresurus stejnegeri lectin (TSL) was expressed in the yeast Pichia pastoris under the control of alcohol oxidase (AOX1) promoter. The recombinant protein, which was glycosylated, can be purified by one-step affinity chromatography. The purified recombinant TSL shared similar sugar-binding activity and Specificity with the native protein as determined by hemagglutination and enzyme-linked lectin binding Assays.
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