Crystallizationand Preliminary X-Ray Crystallographic Studies of Formaldehyde Dehydrogenase from Pseudomonas putida

The formaldehyde dehydrogenease from Pseudomonas putida (PFDH), has been crystallized by the vapour diffusion method using ammonium sulfate as the Precipitating agent. The crystals belong to a trigonal space group P3 12 (or P3 12) with the cell dimensions of a = b = 85.74 A, and c = 190.9 A. There are two subunits per asymmetric unit. The crystals diffract to at least 2.2 A resolution using Cu K Alfa radiation at 100 K. Self-rotation function studies suggest that the tetrameric PFDH molecular has the 222 point group symmetry.