In Vitro Degradation Of beta-Amyloid[25-35] Peptide

Some amyloid-derived peptides show their toxic effects only after a long “aging” period performed several days at pH 7.4 and 37 C for cluster formation. Our experiments show that long aging of beta-amyloid[25-35] under physiological conditions results in peptide bond cleavage between Asn27 and Lys28. Relatively short incubation time (24 hours) and absence of Ca2+ ions prevent peptide cleavage and results in good aggregation of beta-amyloids.