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2000
Volume 32, Issue 9
  • ISSN: 0929-8665
  • E-ISSN: 1875-5305

Abstract

Introduction

The skin of amphibians performs some vital roles, such as camouflage, ion and water transport, and gas exchange. Additionally, it plays a significant role in the immune system by preventing pathogen invasion. The secretions produced by the granular glands in the skin possess antimicrobial properties, which help prevent harmful microorganisms from entering the animal's body. The study aims to determine the total protein amounts in the secretion of (Levant water frog) distributed in Türkiye and to reveal whether it has antimicrobial properties. In this context, it is a pioneering study on antimicrobial peptides in the skin secretion of .

Methods

Field research was carried out at İzmir, Türkiye’s Homeros Valley. Because AMPs are species-specific, not sex-specific peptides, both male and female adults were used, regardless of sex. The average weight of the animals used in the experiment was 21 grams. Granular glands were stimulated using a specialized device, and the secretions were collected for analysis. The collected secretions were lyophilized after centrifugation. Then, the total protein amount in the secretion was determined by Bicinchoninic Acid (BCA). The antibacterial activities of the skin secretions against and were assessed using a plate well diffusion assay. The peptide profiles in the skin secretions were determined using the Tricine-SDS-PAGE electrophoresis method.

Results

The plate well diffusion assay demonstrated that the skin secretions created a 21 mm inhibition zone against and a 20 mm inhibition zone against The results of the Tricine-SDS-PAGE electrophoresis revealed the presence of peptides with five different molecular weights, including one smaller than 5 kDa. As a result of the analysis, it was determined that secretion exhibits antimicrobial properties, and many proteins with different molecular masses were identified in the skin secretion.

Discussion

There are no skin secretion studies on in the literature. However, activity studies were also conducted on the skin secretion of another species, The study examined the antibacterial activities of the skin secretion against some gram-positive and gram-negative bacteria using a plate well diffusion assay. Their study showed that formed a 21 mm zone and formed a 24 mm zone.

Conclusion

This study is preliminary, and in future studies, AMPs in skin secretions can be isolated by chromatographic methods, such as HPLC, and peptides can be sequenced and identified in detail.

© 2025 Bentham Science Publishers
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References

  1. DuellmanW.E. TruebL. Biology of amphibians.Baltimore, MD, USAJHU press199410.56021/9780801847806
     [Google Scholar]
  2. MauricioB. Mailho-FontanaP.L SatoL. A. BarbosaF.F. AstrayR.M. KupferA. 2021Morphology of the cutaneous poison and mucous glands in amphibians with particular emphasis on caecilians (Siphonops annulatus). Toxins2021131177910.3390/toxins13110779
     [Google Scholar]
  3. ToledoR.D. JaredC. Cutaneous granular glands and amphibian venoms.Comp. Biochem. Physiol. Part A: Physiol.19951111110.1016/0300‑9629(95)98515‑I
     [Google Scholar]
  4. DemasG. NelsonR.J. NelsonR. Ecoimmunology.USAOxford University Press2012
     [Google Scholar]
  5. GuoX. LiB. LiangS. LaiR. LiuH. A novel Kunitz-type neuro-toxin peptide identified from skin secretions of the frog Amolops loloensis.Biochem. Biophys. Res. Commun.202052819910410.1016/j.bbrc.2020.05.051
     [Google Scholar]
  6. BarraD. SimmacoM. Amphibian skin: A promising resource for anti-microbial peptides.Trends Biotechnol.199513620510.1016/S0167‑7799(00)88947‑7
     [Google Scholar]
  7. ClarkeB. T. The natural history of amphibian skin secretions, their normal functioning and potential medical applications.Biol. Rev. Camb. Philos. Soc.199772336537910.1017/S0006323197005045
     [Google Scholar]
  8. GomesA. GiriB. SahaA. MishraR. DasguptaS.C. DebnathA. GomesA. Bioactive molecules from amphibian skin: their biological activities with reference to therapeutic potentials for possible drug development.Indian J. Exp. Biol.200745757917821852
     [Google Scholar]
  9. Rollins-SmithL.A. The role of amphibian antimicrobial peptides in protection of amphibians from pathogens linked to global amphibian declines.Biochim. Biophys. Acta Biomembr.2009178881593159910.1016/j.bbamem.2009.03.008
     [Google Scholar]
  10. NicolasP. MorA. Peptides as weapons against microorganisms in the chemical defense system of vertebrates.Annu. Rev. Microbiol.199549127730410.1146/annurev.mi.49.100195.0014258561461
     [Google Scholar]
  11. ConlonJ.M. SonnevendA. DavidsonC. David SmithD. NielsenP.F. The ascaphins: A family of antimicrobial peptides from the skin secretions of the most primitive extant frog, Ascaphus truei.Biochem. Biophys. Res. Commun.2004320117017510.1016/j.bbrc.2004.05.14115207717
     [Google Scholar]
  12. ShahrudinS. IsmailM. KwanS. NajimudinN. Ecology and proteincomposition of Polypedates leucomystax (Gravenhorst, 1829) (Anura: Rhacophoridae) foam nests from Peninsular Malaysia.Annu. Res. Rev. Biol.201714611010.9734/ARRB/2017/34211
     [Google Scholar]
  13. DubosR.J. Studies on a bactericidal agent extracted from a soil bacillus: I. Preparation of the agent. Its activity in vitro.J. Exp. Medicine19397011
     [Google Scholar]
  14. Van EppsH.L. René Dubos: Unearthing antibiotics.J. Exp. Med.20062032259
    [Google Scholar]
  15. DudaT.F.Jr VanhoyeD. NicolasP. Roles of diversifying selection and coordinated evolution in the evolution of amphibian antimicrobial peptides.Mol. Biol. Evolut200219685810.1093/oxfordjournals.molbev.a004143
     [Google Scholar]
  16. ConlonJ.M. MechkarskaM. Host-defense peptides with therapeutic potential from skin secretions of frogs from the family Pipidae.Pharmaceuticals20147158 7710.3390/ph7010058
     [Google Scholar]
  17. HancockR.E. DiamondG. The role of cationic antimicrobial peptides in innate host defences.Trends. Microbiol.20008910210.1016/S0966‑842X(00)01823‑0
     [Google Scholar]
  18. FjellC.D. HissJ.A. HancockR.E.W. SchneiderG. Designing antimicrobial peptides: Form follows function.Nat. Rev. Drug Discov.2012111375110.1038/nrd359122173434
     [Google Scholar]
  19. Rollins-SmithL.A. ReinertL.K. O'LearyC.J. HoustonL.E. WoodhamsD.C. Antimicrobial peptide defenses in amphibian skin.Integrat. Compar. Biol200545113710.1093/icb/45.1.137
     [Google Scholar]
  20. MadiganM.T. MartinkoJ.M. ParkerJ. Brock biology of microorganisms (11th edn).Int. Micob.19978149152
     [Google Scholar]
  21. HorikawaR. ParkerD.S. HerringP.L. PisanoJ.J. Pipinins: New mast cell degranulating peptides from Rana pipiens.Fed. Proc.198544695
     [Google Scholar]
  22. LiJ. Anti-infection peptidomics of amphibian skin.Mol. Cell Proteomics20076588289410.1074/mcp.M600334‑MCP200
     [Google Scholar]
  23. WimleyW.C. HristovaK. Antimicrobial peptides: Successes, challenges and unanswered questions.J. Membr. Biol.20112391-2273410.1007/s00232‑011‑9343‑021225255
     [Google Scholar]
  24. KudiA.C. UmohJ.U. EduvieL.O. GefuJ. Screening of some Nigerian medicinal plants for antibacterial activity.J. Ethnopharmacol.199967222522810.1016/S0378‑8741(98)00214‑110619388
     [Google Scholar]
  25. HaiderS.R. ReidH.J SharpB.L. Tricine-SDS-PAGE.Protein Electrophoresis: Methods and ProtocolsTotowa, NJHumana Press2012819110.1007/978‑1‑61779‑821‑4_8
     [Google Scholar]
  26. VargaJ.F.A. Bui-MarinosM.P. KatzenbackB.A. Frog skin innate immune defences: Sensing and surviving pathogens.Front. Immunol.20199312810.3389/fimmu.2018.0312830692997
     [Google Scholar]
  27. El Haj MoussaA. OlamaZ. MoussadE. KavunjaH. El-DakdoukiH. Characterization of anuran skin peptides: An alternative to the classical therapeutic agents used for MDR pathogens.Int. J. Curr. Microbiol. Appl. Sci.2015416889900
     [Google Scholar]
  28. BayrakciY. Antimicrobial activity of skin secretions obtained from Pelophylax ridibundus. 11th National Ecology and Environment Congress, 01 - 04 OCTOBER 2013, SAMSUN.https://www.researchgate.net/profile/Yusuf-Bayrakci/publication/286778770
  29. SangM. WuQ. XiX. MaC. WangL. ZhouM. BurrowsJ.F. ChenT. Identification and target-modifications of temporin-PE: A novel antimicrobial peptide in the defensive skin secretions of the edible frog, Pelophylax kl. esculentus.Biochem. Biophys. Res. Commun.201849542539254610.1016/j.bbrc.2017.11.17329191658
     [Google Scholar]
  30. FanX.L. YuS.S. ZhaoJ.L. LiY. ZhanD.J. XuF. LinZ.H. ChenJ. Brevinin-2PN, an antimicrobial peptide identified from dark-spotted frog (Pelophylax nigromaculatus), exhibits wound-healing activity.Dev. Comp. Immunol.202213710451910.1016/j.dci.2022.10451936041640
     [Google Scholar]
  31. Tanrıverdi OE. AyazD. TerziY. Comparative analysis of protein profiles in skin secretions of some Rana species: Preliminary insights into antimicrobial activity.Toxicon202425010811010.1016/j.toxicon.2024.10811039343149
     [Google Scholar]
/content/journals/ppl/10.2174/0109298665385364250917100034
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A Preliminary Study on the Antibacterial Activity of the Secretion of the Levantine Water Frog, Pelophylax bedriagae (Camerano, 1882) (Anura:Ranidae)
PPL 32, 650 (2025); https://doi.org/10.2174/0109298665385364250917100034
/content/journals/ppl/10.2174/0109298665385364250917100034
/content/journals/ppl/10.2174/0109298665385364250917100034
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  • Article Type:     Research Article
Keyword(s): Amphibian skin secretion; antimicrobial peptides; Escherichia coli; Pelophylax bedriagae; Staphylococcus aureus; Tricine-SDS-PAGE

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