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- ISSN: 2210-3155
- E-ISSN: 2210-3163
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Harzianolides H-J: Three New Butenolides Isolated from the Fungus Trichoderma harzianum
- Source: Natural Products Journal, The, Volume 16, Issue 3, Mar 2026, e22103155358814
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- 03 Oct 2024
- 07 Nov 2024
- 16 Dec 2024
Abstract
Background
Marine fungi have been recognized as new sources for the discovery of valuable secondary metabolites, providing lead compounds for drug discovery and development.
Objective
The objective of this study was to isolate and identify the secondary metabolites from the marine-derived fungus Trichoderma harzianum ZN-4 and evaluate their antifungal activity.
Methods
Fungus strain Trichoderma harzianum ZN-4 was initially fermentated using a PDB liquid medium. Subsequently, extensive chromatographic methods were applied to isolate the fermentation cultures of ZN-4, and the chemical structures of isolate compounds were elucidated by 1D and 2D NMR, ECD, and HRESIMS. The antifungal activity was evaluated using the broth microdilution method.
Results and Discussion
Three new butenolides, harzianolides H–J (1–3), were isolated from T. harzianum ZN-4, and cultivated on a sterilized PDB medium. Activity evaluation showed that compounds 1-3 exhibited potent antifungal activity with MIC values of 12.5-100 μg/mL against the phytopathogenic fungus Pestalotiopsis theae.
Conclusion
This study not only enriched the structural diversity of the harzianolide family but also revealed their anti-P. theae activity, which may be potential antifungal agents.
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