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2000
Volume 25, Issue 4
  • ISSN: 1871-5265
  • E-ISSN: 2212-3989

Abstract

Background

Microorganisms associated with sea sponges have proven to be good natural product resources that are biologically active and pharmaceutically important.

Objective

This research aimed to identify actinomycetes related to a sponge from Barranglompo Island Makassar and the antibacterial compounds.

Methods

Identification of actinomycetes was based on molecular characterization of sequence gen16S rRNA. The antibacterial compound was separated using vacuum liquid chromatography and preparative Thin Layer Chromatography (TLC). The structure determination was done based on spectroscopy 1H-NMR, 13C-NMR, 2D NMR, and mass spectra.

Results

Molecular characterization showed that actinomycetes strain BLP 20 had the closest relationship with Streptomyces parvulus and Uncultured sp. with a similarity value of 83%. The results obtained from the characterization of antibacterial compounds based on spectroscopic data indicate that these compounds lead to Actinomycin D.

Conclusion

Characterization and identification of Strain 20 / BLP by molecular phylogenetic analysis of 16S rRNA sequences revealed the closest relationship with Uncultured sp and with a similarity value of 83%, which indicated a new species. The structure of the active compound isolated from actinomycetes strain 20 / BLP leads to Actinomycin D.

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Characterization of Antibiotic Actinomycin D Isolated from Streptomyces parvulus Collected from Marine Sponge of Barrang Lompo Island, Makassar, Indonesia
Infect. Disord. Drug Targets 25, E18715265306848 (2025); https://doi.org/10.2174/0118715265306848240719061135
/content/journals/iddt/10.2174/0118715265306848240719061135
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  • Article Type:     Research Article
Keyword(s): 16S rRNA gene; actinomycin D; antibacterial; barrang lompo island; Sponge; streptomyces parvulus

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