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image of Protective Effects of Hydrogen Treatment Against High Glucose-Induced Oxidative Stress and Apoptosis via Inhibition of the AGEs/RAGE/NF-κB Signaling Pathway in Skin Cells
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Abstract

Background

Diabetic wounds are major clinical challenges, often complicated by oxidative stress and free radical generation. Hydrogen (H), a selective antioxidant, offers potential as a therapeutic agent for chronic diabetic wounds. However, its precise mechanisms remain underexplored.

Objective

This study aimed to investigate the protective effects of H on high glucose-induced oxidative damage and apoptosis in human skin cells.

Methods

HaCaT keratinocytes and HSF fibroblasts were treated with high glucose or AGEs. Cell viability, oxidative stress markers, inflammatory cytokines, and apoptosis were analyzed. AGEs/RAGE/NF-κB signaling was evaluated Western blot.

Results

H treatment significantly reduced ROS, MDA, IL-1β, and TNF-α levels, while enhancing SOD and GSH activity. It also inhibited AGEs/RAGE/NF-κB signaling and apoptosis.

Conclusion

Hydrogen therapy protects against oxidative stress and inflammation induced by high glucose or AGEs, offering potential as an adjunctive treatment for diabetic wound healing.

© 2025 The Author(s). Published by Bentham Science Publisher.
This is an open access article published under CC BY 4.0 https://creativecommons.org/licenses/by/4.0/legalcode
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2025-01-08
2025-11-01

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/content/journals/emiddt/10.2174/0118715303369584241231141001
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Protective Effects of Hydrogen Treatment Against High Glucose-Induced Oxidative Stress and Apoptosis via Inhibition of the AGEs/RAGE/NF-κB Signaling Pathway in Skin Cells
Bentham Science Publishers ; https://doi.org/10.2174/0118715303369584241231141001
/content/journals/emiddt/10.2174/0118715303369584241231141001
/content/journals/emiddt/10.2174/0118715303369584241231141001
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  • Article Type:     Research Article
Keywords: human skin cells ; oxidative stress ; Diabetes ; advanced glycation end products ; hydrogen treatment

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