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2000
Volume 22, Issue 2
  • ISSN: 1573-4048
  • E-ISSN: 1875-6581

Abstract

Introduction

Numerous lifestyle factors contribute to the incidence of infertility, which is a major concern for couples throughout the globe. The effectiveness of fertilization (IVF) is highly dependent on the characteristics of the culture media used.

Objective

The present research study aimed to determine the effects of L-aspartic acid, L-ascorbic acid, and silver nanoparticles (AgNPs) on the success rate of IVF in NMRI mice.

Methods

The solutions of L-aspartic acid, L-ascorbic acid, and synthesized AgNPs were prepared. The 6-8 weeks-old NMRI male mice were used as sperm donors, and 6-8 weeks-old NMRI mice were used for oocyte donation. In addition, the IVF procedure was performed using 50 µL drops of KSOM+5%BSA in mineral oil. Seven different experimental groups were investigated in addition to the control group. Oocytes were exposed to sperm for 4-6 h in the KSOM medium as a control group.

Results and Discussion

According to the results, the groups containing supplements “AgNPs”, “L-aspartic acid”, and “AgNPs + L-ascorbic acid”, were not suitable for the 2PN stage. In contrast, the group containing “L-aspartic acid + L-ascorbic acid” was the best group for the 2PN stage. In addition, supplementation of the mixture of L-aspartic acid and AgNPs solution in KSOM+BSA culture medium was the most suitable group for the 2Cell stage.

Conclusion

In conclusion, the study highlighted the varying impacts of L-aspartic acid, L-ascorbic acid, and AgNPs on IVF outcomes in NMRI mice. Group E, combining L-aspartic acid and AgNPs, emerged as effective for 2Cell development. Future research should explore the underlying biochemical mechanisms of these findings. Furthermore, another study is in progress that utilizes these supplements to generate 2PN first, and then the supplement-free medium as the cleavage media.

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2026-03-04

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Effect of the Supplementation of L-Aspartic Acid, L-Ascorbic Acid, and Silver Nanoparticles (AgNPs) to the In vitro Fertilization (IVF) Media on the NMRI Mouse IVF Rates
Curr Womens Health Rev. 22, E15734048363150 (2026); https://doi.org/10.2174/0115734048363150250414112511
/content/journals/cwhr/10.2174/0115734048363150250414112511
/content/journals/cwhr/10.2174/0115734048363150250414112511
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  • Article Type:     Research Article
Keyword(s): 2Cell stage; In vitro fertilization (IVF); L-ascorbic acid; L-aspartic acid; NMRI mouse; silver nanoparticles (AgNPs)

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