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image of A Purified Novel Protein Obtained from Chlorella vulgaris Against Toxoplasma gondii

Abstract

Introduction

This study aimed to investigate the effects of the extract and purified protein from the microalga against in infected Vero cells.

Method

The extract was obtained through magnetic stirring with Tris-HCl buffer and evaluated for cytotoxicity and anti-Toxoplasma activity. The purified protein was isolated using Sephadex G-75 chromatography and assessed in light microscopy assays.

Results

Results indicated that the CC of the extract was > 2000 μg/mL. Both the extract and the purified protein effectively inhibited parasite multiplication, with IC50 values of 132.6 and 8.6 μg/mL, respectively, and selectivity indices of 11.5 and > 20, respectively.

Discussion

Microscopic analysis showed that the purified protein, even at higher concentrations, did not exhibit toxicity to the cells and reduced the number of intracellular tachyzoites.

Conclusion

These findings suggest that both the extract and purified protein of possess the ability to inhibit tachyzoites without causing toxicity to healthy cells, indicating their potential as bioactive compounds for pharmacological applications against toxoplasmosis.

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2026-01-09
2026-01-31

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A Purified Novel Protein Obtained from Chlorella vulgaris Against Toxoplasma gondii
Bentham Science Publishers ; https://doi.org/10.2174/0115680266411952251028115549
/content/journals/ctmc/10.2174/0115680266411952251028115549
/content/journals/ctmc/10.2174/0115680266411952251028115549
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  • Article Type:     Research Article
Keywords: Pharmacological applications ; Natural compound ; Microalgae ; Toxoplasmosis treatment ; Purification process ; Tachyzoite inhibition

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